Effectiveness Modified GMS Staining (GMS-HE, GMS-Phloxine Tatrazine, GMS-Van Gieson and GMS-Masson’s Trichrome) for Detecting Candida Fungus in Biopsy Tissue
Efektivitas Pewarnaan GMS Modifikasi (GMS-HE, GMS-Phloxine Tatrazine, GMS-Van Gieson dan GMS-Masson’s Throchrome) untuk Mendeteksi Jamur Candida pada Jaringan Biopsi
DOI:
https://doi.org/10.21070/ups.6775Keywords:
Candida, Gomori’s Methenamine Silver (GMS), GMS-Haematoxylin & Eosin, GMS-Phloxine Tartrazine, GMS-Van GiesonAbstract
Microscopic examination, histopathology, and fungal staining are essential for diagnosing invasive Candidiasis and other mycoses. Tissue biopsy histology, while more sensitive for detecting fungi, requires special stains like Gomori's Methenamine Silver (GMS). A limitation of GMS staining is its inability to clearly visualize non-fungal structures, necessitating counterstain modifications. This experimental study was conducted at Sudarma Medical Laboratory from March to May 2024, using paraffin-embedded tissues (n=50) with positive and negative controls. Stains included GMS, GMS-Hematoxylin & Eosin, GMS-Phloxine Tartrazine, GMS-Van Gieson, and GMS-Masson's Trichrome, examined at 400x magnification. The Kruskal-Wallis test revealed significant differences between GMS and GMS-Van Gieson (p < 0.05), but not among GMS, GMS-HE, GMS-MT, and GMS-PT (p > 0.05). The findings highlighted that modified GMS-Van Gieson staining provided superior contrast in identifying Candida fungi compared to standard GMS.
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