Analysis of the Inhibitory Efect of Steamed Extract and Infusion of Yellow Squash (Cucurbita moschata) on the Growth of Escherichia coli
Analisis Daya Hambat Ekstrak Kukus dan Infusa Labu Kuning (Cucurbita moschata) Terhadap Pertumbuhan Escherichia coli
DOI:
https://doi.org/10.21070/ups.9598Keywords:
Antibacterial activity, pumpkin flesh, pumpkin seeds, infusion, steamed, Escherichia coliAbstract
Diarrhea is a disease caused by pathogenic microorganism infections, one of which is E.coli, which is a serious problem because it can cause death. One alternative treatment utilizes natural ingredients. This experimental study aims to test the effectiveness of pumpkin (C.moschata) extract processed using the steaming and infusion methods on the growth of E. coli bacteria. The yellow squash samples used were sourced from the Larangan market in Sidoarjo, with criteria of freshness and yellow color. The inhibition test was conducted using the Kirby-Bauer method, repeated three times, with extract concentrations of 25%, 50%, 75%, and 100%, a positive control of chloramphenicol, and a negative control of sterile distilled water. The results showed that all extracts exhibited increased antibacterial activity with increasing concentration, with the largest inhibition zone at a concentration of 100%, namely 12.55 mm for steamed pumpkin flesh and 11.61 mm for steamed pumpkin seeds.
Downloads
References
P. R. Utami, S. Indrayati, And W. Satya, “Kombinasi Ekstrak Lidah Buaya dengan Antibiotik Ciprofloxacin Dapat Menghambat Pertumbuhan Bakteri Escherichia Coli,” J. Kesehat. Perintis (Perintis’s Heal. Journal), Vol. 9, No. 1, Pp. 7–14, 2022, Doi: 10.33653/Jkp.V9i1.772.
S. A. Husna And N. V. Soviadi, “Distribusi Penyakit Diare dan Determinan Dengan Pemetaan Wilayah Provinsi Jawa Barat Tahun 2020,” Vol. 20, No. 2, Pp. 136–146, 2024.
Kementrian Kesehatan, Profil Kesehatan. 2020.
Y. A. Prasetya, I. Y. Winarsih, K. A. Pratiwi, M. C. Hartono, And D. N. Rochimah, “Deteksi Fenotipik Escherichia coli Penghasil Extended Spectrum Beta-Lactamases (Esbls) pada Sampel Makanan di Krian Sidoarjo,” Life Sci., Vol. 8, No. 1, Pp. 95–105, 2019, Doi: 10.15294/Lifesci.V8i1.29995.
D. Anggraini And O. Kumala, “Diare Pada Anak,” Sci. J., Vol. 1, No. 4, Pp. 309–317, 2022, Doi: 10.56260/Sciena.V1i4.60.
W. Nisya Fitri And D. Rahayu, “Review: Aktivitas Antibakteri Ekstrak Tumbuhan Melastomataceae Terhadap Bakteri Escherichia coli dan Staphylococcus aureus,” Farmaka, Vol. 16, Pp. 69–77, 2018.
Y. Safitri, W. R. Gultom, D. A. Tobing, and D. R. Sianturi, “Potensi Escherichia coli Sebagai Resistansi Antibiotik,” Algoritm. J. Mat. Ilmu Pengetah. Alam, Kebumian dan Angkasa, Vol. 2, No. 5, Pp. 8–20, 2024, [Online]. Available: Https://Doi.Org/10.62383/Algoritma.V2i5.109
K.Z.El.A.U.Qumar dan H.M.B. Abdul,“Review :Studies On Bactericidal Peel Efficacy Of Pumpkin (Cucurbita Moschata)” Vol. 2, No. 2, Pp. 146–153, 2014, Doi: 10.12980/Jclm.2.201414j7.
J. N. Agaatsz And M. Sitompul, “Tingkat Pengetahuan dan Sikap Orangtua Berhubungan dengan Penggunaan Obat Tradisional pada Anak,” J. Penelit. Perawat Prof., Vol. 3, No. 2, Pp. 327–338, 2021, Doi: 10.37287/Jppp.V3i2.454.
H. Budiman, R. Supriningrum, And R. Sundu, “Karakterisasi dan Skrining Fitokimia Buah Labu Kuning (Cucurbita moschata),” J. Ris. Kefarmasian Indones., Vol. 6, No. 1, Pp. 16–36, 2024, Doi: 10.33759/Jrki.V6i1.420.
A. Debby Pelu, I. P. Elly, And L. La Bassy, “Skrining Fitokimia dan Uji Aktivitas Ekstrak Etanol Biji Labu Kuning (Curcubita moschata),” J. Sains Dan Kesehat., Vol. 4, No. 1, Pp. 61–70, 2020.
Y. Suria, N. Fitriana, M. Ali, And W. Masrida, “Karakterisasi Senyawa Antibakteri dari Ekstrak Biji Labu Kuning (Cucurbita moschata),” Vol. 3, No. 3, Pp. 214–226, 2024.
M. Muhammad, A. Jamil, I. Institut, I. Pangan, And U. Sargodha, “Antioxidant and Antimicrobial Properties Of Pumpkin (Cucurbita maxima) Peel, Flesh And Seeds Powders,” J. Biol. Agric. Healthc., Vol. 11, No. 6, 2021, Doi: 10.7176/Jbah/11-6-05.
N. Aziz, “Pengaruh Waktu Distilasi Uap Terhadap Rendemen dan Komponen Penyusun Minyak Atsiri Daun Jeruk Nipis (Citrus Aurantifolia (Christm.& Panz.) Swingle ),” 2015, [Online]. Available: Http://Repository.Ub.Ac.Id/Id/Eprint/154357/
D. K. Risfianty And Indrawati, “Perbedaan Kadar Tanin Pada Infusa Daun Asam Jawa (Tamarindus Indica L.) Dengan Metoda Spektrofotometer Uv-Vis,” Lomb. J. Sci., Vol. 2, No. 3, Pp. 1–7, 2020.
I. Lumbantoruan, “Uji Aktivitas Antibakteri Infusa Daun Kesum (Polygonum Minus Huds.) Terhadap Staphylococcus aureus,” J. Chem. Inf. Model., Vol. 53, No. 9, Pp. 1689–1699, 2013, [Online]. Available: File:///C:/Users/User/Downloads/7ed4fa718868545104127281e373061b99cfd8432fb7e1bae73dcc9430f890 36 (1).Pdfkesum
I. A. Putri, M. Fatimura, H. Husnah, And M. Bakrie, “Pembuatan Minyak Atsiri Kemangi (Ocimum Basilicum L.) Dengan Menggunakan Metode Distilasi Uap Langsung,” J. Redoks, Vol. 6, No. 2, Pp. 149– 156, 2021, Doi: 10.31851/Redoks.V6i2.5202.
A. M. Hasanah, K. Kurniawan, And A. Fadholah, “Perbandingan Kadar Total Flavonoid Metode Infusa dan Rendaman Buah Kurma Ajwa ( Phoenix Dactylifera L .) Menggunakan Spektrofotometri Uv-Vis,” J. Ilmah Glob. Farm., Vol. 1, No. 1, Pp. 9–17, 2023.
Rosmania And F. Yanti, “Jurnal Penelitian Sains,” J. Penelit. Sains, Vol. 21, No. 3, Pp. 163–167, 2020, [Online]. Available: Http://Ejurnal.Mipa.Unsri.Ac.Id/Index.Php/Jps/Index
Clsi, M100 Performance Standards For Antimicrobial Suspecbility Testing, Vol. 40. 2020.
D. P. Tilarso, A. Muadifah, W. Handaru, P. I. Pratiwi, And M. L. Khusna, “Aktivitas Antibakteri Kombinasi Ekstrak Daun Sirih dan Belimbing Wuluh Dengan Metode Hidroekstraksi.,” Chempublish J., Vol. 6, No. 2, Pp. 63–74, 2021, Doi: 10.22437/Chp.V6i2.21736.
F. Fauzah, N. Noval, and R. Rohama, “Formulasi Sediaan Serum Dari Ekstrak Labu Kuning (Cucurbita moschata) dengan Variasi Konsentrasi Basis Xanthan Gum Sebagai Antioksidan,” J. Surya Med., Vol. 10, No. 1, Pp. 277–287, 2024, Doi: 10.33084/Jsm.V10i1.7229.
N. I. Firdausi, “Uji Aktivitas Ekstrak Kasar dan Ekstrak Terpurifikasi Biji Labu Kuning (Cucurbita moschata) Terhadap Bakteri Escherichia coli dan staphyllococcus Aureus,” Kaos Gl Derg., Vol. 8, No. 75, Pp. 147–154, 2020, [Online]. Available: Https://Doi.Org/10.1016/J.Jnc.2020.125798%0ahttps://Doi.Org/10.1016/J.Smr.2020.02.002%0ahttp://Www .Ncbi.Nlm.Nih.Gov/Pubmed/810049%0ahttp://Doi.Wiley.Com/10.1002/Anie.197505391% 0ahttp://Www.S ciencedirect.Com/Science/Article/Pii/B9780857090409500205%0ahttp:
I. Sunnah, G. A. Fadiyah, W. A. Silmi, And A. R. Erwiyani, “Potensi Antibakteri Minyak Biji Labu Kuning (Cucurbita moschata) Terhadap Penghambatan Bakteri Gram Positif Antibacterial Potential Of Yellow Pumpkin ( Cucurbita Moschata D ) Seed Oil Against Inhibition Of Gram-Positive Bacteria,” Vol. 9, No. 2, Pp. 4–9, 2024, Doi: 10.18860/Jip.V9i2.27010.
S. Wahyuni Et Al., “Edukasi Pembuatan Media Nutrient Agar (Na) Untuk Pengamatan Morfologi Esherichia coli Di Smas Pesantren Immim,” Vol. 5, No. 1, Pp. 31–36, 2024.
L. Khakim And C. S. Rini, “Identifikasi Eschericia Coli Dan Salmonella Sp. Pada Air Kolam Renang Candi Pari,” Medicra (Journal Med. Lab. Sci., Vol. 1, No. 2, Pp. 84–93, 2018, Doi: 10.21070/Medicra.V1i2.1491.
P. Standards And A. S. Testing, M100 Performance Standards For Antimicrobial, Vol. 40, No. 1. 2020.
R. Dian, . F., And F. Budiarso, “Uji Resistensi Bakteri Escherichia coli Yang Diisolasi dari Plak Gigi Terhadap Merkuri dan Antibiotik Kloramfenikol,” J. E-Biomedik, Vol. 3, No. 1, 2015, Doi: 10.35790/Ebm.3.1.2015.6607.
A. Kuswandi, K. Kusmiyati, And H. Holikin, “Efektivitas Kompres Iodine Terhadap Zona Hambat Staphylococcus Aureus Pada Ulkus Diabetikum,” J. Keperawatan Indones., Vol. 16, No. 3, Pp. 139–144, 2013, Doi: 10.7454/Jki.V16i3.323.
M. M. Sari, M. Idrus, And D. R. Trisnapatri, “Uji Sensitivitas Antibiotika Cefixime Dan Levofloxacin Terhadap Bakteri Staphylococcus Aureus Penyebab Ispa Sensitivity Testing Of Cefixime And Levofloxacin Antibiotics The Bacteria Staphylococcus Aureus Causes Acute Respiratory Tract Infections,” Vol. 4, No. 2, 2025.
I. D. Widyanti And M. Maryati, “Shigella Sonnei And Bacillus Cereus Bacteria,” Usadha J. Pharm., Vol. 2, No. 1, Pp. 60–71, 2023, [Online]. Available: Https://Jsr.Lib.Ums.Ac.Id/Index.Php/Ujp
F. S. Emmaputri., “Kajian Poses Destilasi Fraksinasi Biodiesel Kemiri Sunan ( Reutealis Trisperma ),” Vol. 12, No. 2, 2018.
Aulia Debby Pelu, Ira P. Ely, And Lukman La Bassy, “Skrining Fitokimia Dan Uji Aktivitas Ekstrak Etanol Biji Labu Kuning (Curcubita Moschata) Terhadap Daya Hambat Bakteri Staphylococcus Aureus,” J. Sains Dan Kesehat., Vol. 4, No. 1, Pp. 61–70, 2020, Doi: 10.57214/Jusika.V4i1.151.
Rusyda Nur Adilah, “Pengaruh Pemberian Ekstrak Daun Sembukan (Paederia Foetida L) Terhadap Bakteri Pseudomonas Fluorescens Secara In Vitro”. Skripsi. 2019.
P. Astuti, R. Rohama, And S. Budi, “Profil Kromatografi Dan Penentuan Kadar Flavonoid Total Fraksi NHeksan Daun Kalangkala (Litsea Angulata Bl) Menggunakan Spektrofotometri Uv-Vis,” J. Pharm. Care Sci., Vol. 3, No. 2, Pp. 30–41, 2023, Doi: 10.33859/Jpcs.V3i2.237.
I. Rosyada, I. Tivani, And W. Amananti, “Uji Antibakteri Sabun Nanopartikel Dengan Ag Ekstrak Daun Turi (Sesbania Grandiflora) dengan Metode Dilusi,” Justek J. Sains Dan Teknol., Vol. 6, No. 4, Pp. 581–592, 2023, [Online]. Available: Http://Journal.Ummat.Ac.Id/Index.Php/Justek
Downloads
Additional Files
Posted
License
Copyright (c) 2025 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
