Identification Of Transcription Factor 7 Like 2 (TCF7L2) SNP Rs7901695 Gene Mutations in Type 2 Diabetes Mellitus Patients Using Direct Polymerase Chain Reaction
Identifikasi Mutasi Gen Transcription factor 7 like 2 (TCF7L2) SNP rs7901695 pada Penderita Diabetes Melitus Tipe-2 Menggunakan Polymerase Chain Reaction Secara Langsung
DOI:
https://doi.org/10.21070/ups.8349Keywords:
Type-2 Diabetes Mellitus, TCF7L2, SNPrs7901695, direct PCR, genetic mutationAbstract
Type-2 Diabetes Mellitus (T2DM) is a chronic metabolic disorder influenced by genetic factors, including mutations in the TCF7L2 gene, particularly the SNP rs7901695. This study aims to detect the rs7901695 mutation in T2DM patients using a direct Polymerase Chain Reaction (PCR) method that is simple and cost-effective. A total of 30 blood samples from T2DM patients were analyzed using allele-specific primers targeting T allele (177 bp) and C allele (367 bp). Most patients were in the 50-59 years age group, indicating the importance of early detection during productive age. Electrophoresis results showed that 28 samples (93.3%) had the heterozygous TC genotype and 2 samples (6.7%) had the homozygous TT genotype, with no CC genotype found.. In conclusion, direct PCR is a practical and efficient method for early genetic identification in T2DM and may support personalized preventive strategies based on genetic screening.
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