Screening Methichillin Resistant Staphylococcus Aureus (MRSA) from Pus Samples in Patients with Tissue Injuries
Screening Methichillin Resistant Staphylococcus Aureus (MRSA) dari Sampel Pus pada Pasien Penderita Luka Jaringan
DOI:
https://doi.org/10.21070/ups.6717Keywords:
Methichillin Resistant Staphylococcus aureus, MRSA, PusAbstract
Staphylococcus aureus is a bacterium commonly found on various parts of the human body. Some variants of Staphylococcus aureus are resistant to ß-lactam and methicillin antibiotics; are known as Methicillin-resistant Staphylococcus aureus (MRSA). The aim of this study is to screen for MRSA in pus samples from patients with tissue wounds. This study employs a descriptive analysis design. The research was conducted from March to June 2024 at the Microbiology Laboratory of Dr. Saiful Anwar Hospital. A total of 44 pus samples were used, with the inclusion criteria being pus samples from inpatients with purulent wounds. The samples were then cultured on Blood Agar Plates (BAP) and subjected to Gram staining to identify the bacterial type. Catalase and coagulase tests were performed to determine the species, followed by antibiotic susceptibility testing using the Vitek 2 compact system. The results of this study revealed a 50% colonization rate of MRSA bacteria.
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D. Erikawati, D. Santosaningsih, and S. Santoso, “Tingginya Prevalensi MRSA pada Isolat Klinik Periode 2010- 2014 di RSUD Dr. Saiful Anwar Malang, Indonesia,” Jurnal Kedokteran Brawijaya, vol. 29, no. 2, pp. 149–156, 2016, doi: 10.21776/ub.jkb.2016.029.02.9.
S. F. Widiyastuti, “Identifikasi Bakteri Methicilin-Resistant Staphylococcus Aureus (Mrsa) Dan Vancomicyn-Resistant Staphylococcus aureus (VRSA) Pada Pasien Infeksi Luka Operasi (ILO) di RSUD DR.H. ABDUL MOELOEK Bandar Lampung,” Fakultas Kedokteran Universitas LampungFakultas Kedokteran Universitas Lampung, 2023.
R. P. Sholecha, E. Yunitasari, N. K. A. Armini, and Y. S. Arief, “Analisis Faktor yang berhubungan dengan Pencegahan Stunting pada Anak Usia 2-5 Tahun berdasarkan Teori Health Promotion Model (HPM),” Pediomaternal Nursing Journal, vol. 5, no. 1, p. 49, 2019, doi: 10.20473/pmnj.v5i1.12362.
A. Fitria, D. E. Widiasi, and H. Airlangga, “Systematic Literature Review: Prevalensi Methicillin-Resistant Staphylococcus Aureus (Mrsa) Terhadap Infeksi Nosokomial di Beberapa Negara Asia,” Jurnal Kedokteran Komunitas, vol. 9, no. 1, pp. 1–8, 2021.
A. Nuryah, N. Yuniarti, and I. Puspitasari, “Prevalensi dan Evaluasi Kesesuaian Penggunaan Antibiotik pada Pasien dengan Infeksi Methicillin Resistant Staphylococcus Aureus di RSUP Dr. Soeradji Tirtonegoro Klaten,” Majalah Farmaseutik, vol. 15, no. 2, p. 123, 2019, doi: 10.22146/farmaseutik.v15i2.47911.
A. Disya Salsabil, M. Rochmanti, and A. Dwi Wahyu Widodo, “Vancomycin Monotherapy vs Alternative Antibiotics for MRSA Patients: A Systematic Review,” International Journal of Research Publications, vol. 92, no. 1, pp. 124–135, 2021, doi: 10.47119/ijrp100921120222689.
I. W. Rahman, N. Arfani, and J. V. Tadoda, “Deteksi Bakteri MRSA Methicillin-Resistant Staphylococcus aureus pada Sampel Darah Pasien Rawat Inap,” Jurnal Ilmu Alam dan Lingkungan, vol. 14, no. 1, pp. 48–54, 2023.
L. Thimmappa et al., “Risk factors for wound infection caused by methicillin resistant staphylococcus aureus among hospitalized patients: A case control study from a tertiary care hospital in India,” Afr Health Sci, vol. 21, no. 1, pp. 286–294, 2021, doi: 10.4314/ahs.v21i1.37.
Naziyah, R. Hidayat, and Maulidya, “Penyuluhan Manajemen Luka Terkini dalam Situasi Pandemic Covid - 19 Melalui Kegiatan Pesantren Luka dengan Menggunakan Media Zoom Meeting bagi Mahasiswa Prodi Keperawatan & Profesi Ners Fakultas Ilmu Kesehatan Universitas Nasional Jakarta,” JURNAL KREATIVITAS PENGABDIAN KEPADA MASYARAKAT (PKM), vol. 2, no. 1, pp. 1–4, 2022.
D. Santosaningsih et al., “Prevalence and characterisation of Staphylococcus aureus causing community-acquired skin and soft tissue infections on Java and Bali, Indonesia,” Tropical Medicine and International Health, vol. 23, no. 1, pp. 34–44, 2018, doi: 10.1111/tmi.13000.
Sugiyono, Metode peneitian kuantitatif, kualitatif dan R&D. Bandung: Alfabeta, 2019.
T. Lasmini, A. Saphira, L. B. Dos Marliana, and T. Sherly Margaretta, “Identifikasi Bakteri Staphylococcus Aureus Pada Swab Rongga Hidung Penjamah Makanan Di Jalan Durian Kota Pekanbaru,” Prosaiding AIPLMI, vol. 5, pp. 281–292, 2022.
A. C. Westgeest et al., “The association of female sex with management and mortality in patients with Staphylococcus aureus bacteraemia,” Clinical Microbiology and Infection, vol. 29, no. 9, pp. 1182–1187, 2023, doi: 10.1016/j.cmi.2023.06.009.
E. Mitevska, B. Wong, B. G. J. Surewaard, and C. N. Jenne, “The prevalence, risk, and management of methicillin-resistant Staphylococcus aureus infection in diverse populations across Canada: A systematic review,” Pathogens, vol. 10, no. 4, 2021, doi: 10.3390/pathogens10040393.
H. Humphreys, F. Fitzpatick, and B. J. Harvey, “Gender differences in rates of carriage and bloodstream infection caused by methicillin-resistant staphylococcus aureus: Are they real, do they matter and why?,” Clinical Infectious Diseases, vol. 61, no. 11, pp. 1708–1714, 2015, doi: 10.1093/cid/civ576.
R. Mahmudah, T. U. Soleha, and C. Ekowati, “Identifikasi Methicillin-Resistant Staphylococcus Aureus (MRSA) Pada Tenaga Medis Dan Paramedis Di Ruang Intensivecare Unit (ICU) Dan Ruang Perawatan Bedah Rumah Sakit Umum Daerah Abdul Moeloek,” Medical Journal of Lampung University, vol. 2, no. 4, pp. 70–78, 2013.
N. R. Waterlow, B. S. Cooper, J. V. Robotham, and G. M. Knight, “Antimicrobial resistance prevalence in bloodstream infection in 29 European countries by age and sex: An observational study,” PLoS Med, vol. 21, no. 3, pp. 1–24, 2024, doi: 10.1371/journal.pmed.1004301.
M. Falcone and G. Tiseo, “Skin and soft tissue infections in the elderly,” Curr Opin Infect Dis, vol. 36, no. 2, pp. 102–108, 2023, doi: 10.1097/QCO.0000000000000907.
C. A. Simanjuntak, “Identification of SCC MEC Methicillin-Resistant Staphylococcus Aureus ( MRSA ) From Hospitals ’ Clinical Samples in Jambi using Poly merase Chain Reaction ( PCR ) concern throughout the world and associated with infection in both Hospital-acquired Methici,” vol. 8, no. 2, 2020.
Prisma Anjarlena, Suliati, Wisnu Istanto, and Retno Sasongkowati, “Detection of Panton-Valentine Leukocidin (Pvl) Gene Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) in Diabetic Ulcer Patients,” Journal of Vocational Health Studies, vol. 7, no. 1, pp. 17–23, 2023, doi: 10.20473/jvhs.v7.i1.2023.17-23.
A. Fitria, “The Bactericidal and Antibiofilm Activity of Stem Bark of Jatropha multifida L. Against Staphylococcus aureus and MRSA,” EKSAKTA: Journal of Sciences and Data Analysis, vol. 18, pp. 42–55, 2018, doi: 10.20885/eksakta.vol18.iss1.art5.
A. Sekar, “Isolasi Dan Identifikasi Bakteri Stahylococcus aureus dari Pus Infeksi Luka Operasi di RSUP.Dr. M. DJAMIL Padang,” Universitas Perintis Indonesia, 2023.
A. Setiawati, “Peningkatan Resistensi Kultur Bakteri Staphylococcus aureus terhadap Amoxicillin Menggunakan Metode Adaptif Gradual,” Jurnal Farmasi Indonesia ■, vol. 7, no. 3, pp. 190–194, 2015.
A. H. Hasanpour et al., “The global prevalence of methicillin-resistant Staphylococcus aureus colonization in residents of elderly care centers: a systematic review and meta-analysis,” Antimicrob Resist Infect Control, vol. 12, no. 1, pp. 1–11, 2023, doi: 10.1186/s13756-023-01210-6.
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