Comparison of PAS and GMS Conventinal Staining with Modification (PAS-Tartrazine and GMS-Phloxine Tartrzaine) on Aspergillus and Chromoblastomycosis Fungi in Tissues
Perbandingan Pewarnaan PAS dan GMS Konvensional dengan Modifikasi (PAS-Tartrazine dan GMS-Phloxine Tartrazine) pada Jamur Aspergillus dan Chromoblastomycosis dalam Jaringan
DOI:
https://doi.org/10.21070/ups.5486Keywords:
Aspergillus, Chromoblastomycosis, PAS, GMS, PAS-Tartrazine, GMS-Phloxine TartrazineAbstract
Fungal infections can occur in the lungs due to Aspergillus or in subcutaneous tissue caused by Chromoblastomycosis. Histopathological staining of fungi uses Periodic Acid Schiff (PAS) and Gomori's Methenamine Silver (GMS). The purpose of this study is to compare the staining quality between conventional PAS and GMS with modified (PAS-Tartrazine and GMS-Phloxine Tartrazine) on Aspergillus and Chromoblastomycosis in tissues. The study design is descriptive exploratory. The research was conducted at Sudarma Medical Laboratory, Surabaya in May 2024. The research samples were paraffin blocks and stained using PAS, GMS, PAS-Tartrazine, and GMS-Phloxine Tartrazine. The number of samples was 6 with 2 repetitions. The staining quality assessment results were tested with the Mann-Whitney test. In PAS and PAS-Tartrazine staining a sig. value <0.05, where in GMS and GMS-Phloxine Tartrazine staining a sig. value <0.05. In PAS-Tartrazine, the fungi appeared clearer compared to PAS, while in GMS and GMS-Phloxine Tartrazine, appeared equally clear
Downloads
References
F. Puspitasari and A. P. Kawilarang, “Perbandingan Pewarnaan Periodic Acid Schiff (PAS) McManus dan Gomori’s Methenamine Silver (GMS)-Phloxine Tartrazine pada Infeksi Jamur (Candidiasis, Basidiobolomycosis, Mucormycosis) dalam Jaringan,” Jurnal Mikologi Klinik dan Penyakit Menular (JMKPM ), vol. 1, no. 1, pp. 1–5, 2023.
C. Firacative, “Invasive fungal disease in humans: Are we aware of the real impact?,” Mem Inst Oswaldo Cruz, vol. 115, no. 9, pp. 1–9, 2020, doi: 10.1590/0074-02760200430.
F. Bongomin, S. Gago, R. O. Oladele, and D. W. Denning, “Global and multi-national prevalence of fungal diseases—estimate precision,” Journal of Fungi, vol. 3, no. 4, 2017, doi: 10.3390/jof3040057.
M. R. Lee et al., “Seroprevalence of Aspergillus IgG and disease prevalence of chronic pulmonary aspergillosis in a country with intermediate burden of tuberculosis: a prospective observational study,” Clinical Microbiology and Infection, vol. 26, no. 8, pp. 1091.e1-1091.e7, 2020, doi: 10.1016/j.cmi.2019.12.009.
A. Rozaliyani et al., “Infeksi Jamur Paru Indonesia: Situasi Saat Ini dan Tantangan di Masa Depan,” J Respir Indo, vol. 39, no. 3, pp. 210–214, 2019.
S. Sasmanto, P. D. Endraswari, and D. Kusumaningrum, “Neglected tropical disease-chromoblastomycosis : a simple KOH staining for definitive diagnosis,” Bali Medical Journal, vol. 12, no. 3, pp. 10–12, 2023, doi: 10.15562/bmj.v12i3.5000.
A. Pohan and Kawilarang, “Perbandingan Pengecatan GMS-Phloxine Tartrazine dengan Pengecatan Hematoxylin & Eosin (H&E) dan Pengecatan PAS-Tartrazine pada jamur Mycetoma,” Jurnal Mikologi Klinik dan Penyakit Menular ( JMKPM ), vol. 1, no. 1, pp. 1–5, 2022.
A. Pohan. Kawilarang, “Perbandingan Pengecatan GMS-Phloxine Tartrazine pada Pneumocystis carinii dengan Pengecatan Gomori Methenamine Silver (GMS) dan Pengecatan Haematoxylin & Eosin (H&E),” Jurnal Mikologi Klinik dan Penyakit Menular ( JMKPM ), vol. 1, no. 1, pp. 1–5, 2022.
F. Puspitasari and A. P. Kawilarang, “Perbandingan Pewarnaan Periodic Acid Schiff (PAS) McManus dan Gomori’s Methenamine Silver (GMS)-Phloxine Tartrazine pada Infeksi Jamur (Candidiasis, Basidiobolomycosis, Mucormycosis) dalam Jaringan Fransisca,” Jurnal Mikologi Klinik dan Penyakit Menular (JMKPM) Vol., vol. 2, no. 1, pp. 16–23, 2023.
M. Octora, A. P. Kawilarang, and N. Hasbi, “Challenge of histopathological method to diagnose double cutaneous invasive fungal infection in Indonesia : a rare pediatric malignancy case report,” vol. 12, no. 3, pp. 24–26, 2023, doi: 10.15562/bmj.v12i3.5000.
K. S. Suvarna, C. Layton, and J. D. Bancroft, Bancroft’s Theory and Practice of Histological Techniques 8th Edition, Eighth. Elsevier Ltd, 2018.
R. Handriani and N. Latifah, “Pewarnaan Jamur Aspergilus. Sp dengan Pewarna Alternatif dari Ekstrak BUnga Telang (Clitoria Ternatea),” Jurnal Analisa Biologi, vol. 7, no. 1, pp. 26–30, 2023.
A. Pohan. Kawilarang, “Perbandingan Pewarnaan Periodic Acid Schiff (PAS) dan Gomori Methenamine Silver (GMS) Pada Pasien Tinea versicolor,” Jurnal Mikologi Klinik dan Penyakit Menular (JMKPM), vol. 1, no. 1, pp. 21–23, 2022.
A. P. Kawilarang, “Perbandingan Pewarnaan Periodic Acid Schiff(PAS) dan Gomori Methenamine Silver(GMS) pada Jamur dalam Jaringan,” Jurnal Mikologi Klinik dan Penyakit Menular (JMKPM), vol. 1, no. 1, pp. 1–5, 2022.
O. O. Adegoke, A. E. Ajao, and G. H. Ano-Edward, “Congenital infantile digital fibromatosis: A case report and review of the literature,” Afr Health Sci, vol. 20, no. 4, pp. 1865–1869, 2020, doi: 10.4314/ahs.v20i4.42.
Downloads
Additional Files
Posted
License
Copyright (c) 2024 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
