The Effect of 10 % Formalin Buffer Fixation Duration on The Quality Of Hematoksilin Eosin (HE) Staining in Tissue Suspected of Breast Cancer
Pengaruh Durasi Fiksasi Buffer Formalin 10 % Terhadap Kualitas Pewarnaan Hematoksilin Eosin (HE) pada Jaringan Terduga Kanker Payudara
DOI:
https://doi.org/10.21070/ups.5338Keywords:
Breast cancer, duration fixation of buffer formalin 10%, Hematoksilin EosinAbstract
Histopathological examination plays an important role in providing an accurate diagnosis of breast cancer. Fixation is the most important factor in the initial procedure of histopathologic examination. The purpose of this study was to determine the optimal duration of fixation of 10% formalin buffer against suspected breast cancer tissue. This study used an analytical descriptive design, tissue samples suspected of breast cancer were fixed for 1 day, 3 days, 7 days. The research was conducted at the Anatomical Pathology Laboratory of dr. Saiful Anwar Hospital, East Java Province, in May 2024 with a total of 9 samples and 2 repetitions. Hematoksilin Eosin staining was carried out automaticall using a Gemini ASS. The staining of quality assessment data was tested with the Kruskal Wallis test shows sig. value of 0.000 (< 0.05), indicating a significant difference between 1 day and 3 days, as well as between 1 day and 7 days
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