Acute Toxicity Test of White Turi Flower (Sesbania grandiflora (L.) Pers.) Ethanol Extract on SGOT and SGPT Parameters in the Liver of Wistar Strain Male White Rats (Rattus norvegicus)
Uji Toksisitas Akut ekstrak Etanol Bunga Turi Putih (Sesbania grandiflora (L.) Pers.) Terhadap Parameter SGOT dan SGPT pada Organ Hati Tikus Putih Jantan (Rattus norvegicus) Galur Wistar
DOI:
https://doi.org/10.21070/ups.2620Keywords:
acute toxicity, white turi flowerAbstract
Turi plants contain phenolic compounds, tannins, flavonoids, alkaloids, and glycosides. In addition, turi plants also contain potentially toxic compounds such as pyrrolizidine compounds. The purpose of this study was to determine the acute toxicity of ethanol extract of white turi flowers on toxic symptoms, macroscopic observations of liver organs, and measurement of SGOT and SGPT levels. The samples of this study were divided into 4 groups, namely normal control, doses of 10,000, 15,000, and 20,000 mg/kgBB, then observed toxic symptoms, liver macroscopic, and SGOT-SGPT levels. The results of macroscopic observations of liver organs showed normal conditions. Statistical test results of SGOT and SGPT levels showed that there were significant differences in each group. Based on the results of the study, it can be concluded that the toxicity of ethanol extract of white turi flowers can affect the increase in SGPT and SGPT levels.
Downloads
References
J. Rohmah, N. R. Rachmawati and S. Nisak, “Perbandingan Daya Antioksidan Ekstrak Aseton Daun dan Batang Turi Putih (Sesbania grandiflora) dengan Metode DPPH (diphenilpycrylhydrazil)”. Prosiding Seminar Nasional Hasil Riset dan Pengabdian (SNHRP-1), pp. 665-677, Desember 2018. [Online] Available: http://eprints.umsida.ac.id/5927/. [Accessed: Juni, 26, 2023].
Sumayya, “Uji Toksisitas Akut Ekstrak Air Daun Turi (Sesbania grandiflora (L).Pers) pada Embrio Ikan Zebra (Danio rerio)”. Skripsi. Program studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia. Yogyakarta, 2010. [Online] Available: https://dspace.uii.ac.id/handle/123456789/17000?show=full. [Accessed: Juni, 26, 2023].
H. Wiedenfeld, “Plants Containing Pyrrolizidine Alkaloids: Toxicity and Problems”. Journal Food Additives and Contaminants, vol. 28, no.3, pp. 282–292, Februari 2011. [Online]. Doi: https://doi.org/10.1080/19440049.2010.541288. [Accessed: Juni, 26, 2023].
H. S. Wicaksono, I. Narayani and I. Setyawati, “Struktur Hati Mencit (Mus musculus L.) setelah Pemberian Ekstrak Daun Kaliandra Merah (Calliandra calothyrsus Meissn.)”. Jurnal Simbiosis III, 1, pp 258-268, Maret 2015. [Online] Available: https://ojs.unud.ac.id/index.php/simbiosis/article/download/14405/9901. [Accessed: Juni, 26, 2023].
M. A. Mustapa, T. S. Tuloli and A. M. Mooduto, “Uji Toksisitas Akut yang Diukur dengan Penentuan LD50 Ekstak Etanol Cengkeh (Syzygium aromaticum L.) terhadap Mencit (Mus musculus) Menggunakan Metode Thompson-Weil”. Jurnal Frontiers. vol 1, no. 1, pp. 105-117, April 2018. [Online] Available: http://ejournal.unima.ac.id/index.php/efrontiers/article/view/221. [Accessed: Juni, 26, 2023].
Jumain, Syahruni and F. T. Farid, “Uji Toksisitas Akut dan LD50 Ekstrak Etanol Daun Kirinyuh (Euphatorium odoratum Linn) pada Mencit (Mus musculus)”. Media Farmasi, vol. 14 no. 1, pp. 28-34, April 2018. [Online] Available: https://journal.poltekkes-mks.ac.id/ojs2/index.php/mediafarmasi/article/view/82/44. [Accessed: Juni, 26, 2023].
M. Hasanah, “Uji Toksisitas Ekstrak Kulit Batang Turi putih (Sesbania grandiflora (L.) Pers.) Menggunakan Pelarut Etanol Terhadap Organ Hati (SGOT-SGPT) Mencit (Mus musculus)”. Skripsi, Program studi Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah. 2022. [Online] Available: http://ais.umsida.ac.id/eskripsi/?h=abstrak&id=7222. [Accessed: Juni, 26, 2023].
M. Arunabha and N. Satish, “Evaluation of Immunomodulatory Activity of Sesbania grandiflora Flowers Extract in Mice. Indonesian journal of Pharmacy, vol. 25, no. 4, pp. 277-283, 2014. [Online]. Doi: 10.14499/indonesianjpharm25iss4pp277. [Accessed: Juni, 26, 2023].
A. Wijaya and Noviana, “Penetapan Kadar Air Simplisia Daun kemangi (Ocimum basilicum L.) Berdasarkan Perbedaan Metode Pengeringan”. Jurnal Riset Kefarmasian Indonesia, vol. 4, no. 2, pp. 185-194, 2022. [Online]. Doi: https://doi.org/10.33759/jrki.v4i2.246. [Accessed: Juni, 28, 2023].
H. Wijaya, S. Jubaidah and Rukayyah, “Perbadingan Metode Ekstraksi Maserasi dan Sokhletasi terhadap Rendemen Ekstrak Batang Turi (Sesbania grandiflora L.)”. Indonesian Journal of Pharmacy and Natural Product, vol. 05 no. 01, pp. 1-11, Maret 2022. [Online]. Doi: https://doi.org/10.35473/ijpnp.v5i1.1469. [Accessed: Juni, 28, 2023].
H. Ih, I. Fajriaty, S. P. Rahmawani and Abdurrachman, “Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis dari Ekstrak Etanol Herba Pacar Air ( Impatiens balsamina Linn)”. Seminar Nasional Pendidikan MIPA dan Teknologi IKIP PGRI Pontianak. 2017. [Online]. Available: https://journal.ikippgriptk.ac.id. [Accessed: Juni, 28, 2023].
M. Nainggolan, S. Ahmad, D, Pertiwi and S. E. Nugraha, Penuntun dan laporan praktikum fitokimia. Medan: Universitas Sumatera Utara”. 2019. [Online]. Available https://ffar.usu.ac.id. [Accessed: Juni, 28, 2023].
D. E. P. Prayoga, K. A. Nocianitri and N. N. Puspawati, “Identifikasi Senyawa Fitokimia dan Aktivitas Antioksidan Ekstrak Kasar Daun Pepe (Gymnema reticulatum Br) pada Berbagai Jenis Pelarut”. Jurnal Ilmu Dan Teknologi Pangan, vol. 8, no. 2, pp. 11-121, Juni 2019. [Online] Doi: https://doi.org/10.24843/itepa.2019.v08.i02.p01. [Accessed: Juni, 28, 2023].
A. I. Habibi, R. A. Firmansyah and S. M. Setyawati, “Skrining Fitokimia Ekstrak n-Heksana Kosteks Batang Salam (Syzgium poluanthum)”. Indo. J. Chem. Sci, vol. 7,no. 1, pp. 1-4, Mei 2018. [Online] Doi: 10.15294/ijcs.v7i1.23370. [Accessed: Juni, 28, 2023].
F. N. S. Datu, Hasriand D. E. Pratiwi, “Identifikasi dan Uji Kestabilan Tanin dari daging Biji Pangi (Pangium edule Reinw.) sebagai bahan Pewarna Alami”. Jurnal Chemica, vol. 22, no. 1, pp. 29-34, Juni 2021. [Online] Doi: https://doi.org/10.35580/chemica.v22i1.21726. [Accessed: Juni, 28, 2023].
C. S. Sulastra, K. Khaerati and Ihwan, “Toksisitas Akut Dan Lethal Dosis (LD50) Ekstrak Etanol Uwi Banggai Ungu (Dioscorea alata L.) Pada Tikus Putih (Rattus norvegicus). Jurnal Ilmiah Medicamento, vol. 6 no. 1, pp. 10-14.2020. [Online] Doi: https://doi.org/10.36733/medicamento.v6il.715. [Accessed: Juni, 29, 2023].
Badan Pengawas Obat dan Makanan Republik Indonesia (BPOM RI), Peraturan BPOM Nomor 10 Tahun 2022 tentang Pedoman uji toksisitas praklinik secara in vivo. Jakarta: Kepala Pengawas Obat dan Makanan Republik Indonesia. 2022. [Online]. Available: https://peraturan.bpk.go.id/. [Accessed: Juni, 29, 2023].
F. Ubang, V. O. Siregar and Herman, “Efek Toksik Ekstrak Etanol Daun Mekai (Albertisia papuana Becc.) terhadap Mencit. Proc. Mul. Pharm. Conf, pp. 49-57, November 2022. [Online] Doi: https://doi.org/10.25026/mpc.v16i1.672. [Accessed: Juni, 29, 2023].
A. N. Febrika, “Uji Toksisitas Akut Kombinasi Ekstrak Etenaol Herba Pegagan (Centella asiantica (L.) Urban), Herba Sambiloto (Andographis paniculata (Burm.F.) Ness), dan Rimpang Temu Giring (Curcuma heyneana valeton & Zipj) pada Tikus”. Skripsi, Universitas Sumatera Utara Medan. 2021 [Online]. Available: http://repository.usu.ac.id/handle/123456789/37226?show=full. [Accessed: Juni, 29, 2023].
A. N. Hasana, A. J. Sitasiwi and S. S. Isdadiyanto, “Hepatosomatik Indeks dan Diameter Hepatosit Mencit (Mus musculus L.) Betina Setelah Paparan Ekstrak Etanol Daun Mimba (Azadirachta indica Juss.)”. Jurnal Pro-Life, vol. 6, no. 1, pp. 1-12, Maret 2019. [Online] Doi: https://doi.org/10.33541/jpvol6Iss2pp102. [Accessed: Juni, 29, 2023].
A. Rosida. “Pemeriksaan Laboratorium Penyakit Hati”. Berkala Kedokteran, vol. 12, no. 1, pp. 123-131, 2016. [Online] Doi: http://dx.doi.org/10.20527/jbk.v12i1.364 [Accessed: Juli, 06, 2023].
S. Purwaningsih, E. Handharyan and I. R. Lestari. “Pengujian Toksisitas Sub Akut Ekstrak Hipokotil Bakau Hitam pada Tikus Galur Spragur Dawley”. Jurnal Akuatika, vol. VI, no. 1, pp. 30-40, Maret 2015. [Online] Available: http://jurnal.unpad.ac.id/akuatika/article/view/5962. [Accessed: Juli, 06, 2023].
Schrenk D, Gao L, Lin G, Mahony C, Mulder PPJ, Peijnenburg A, Pfuhler S, Rietjens IMCM, Rutz L, B, “These A. Pyrrolizidine alkaloids in food and phytomedicine: Occurrence, exposure, toxicity, mechanisms, and risk assessment - A review. Food Chem Toxicol. 2020 Feb;136:111107. doi: 10.1016/j.fct.2019.11110 [Accessed: Juli, 11, 2023].
F. N. Aulia. “Uji Toksisitas Subkronik Kombinasi Esktrak Etanol Daun Mangga Pakel dengan Daun Pandan Wangi terhadap Kadar SGOT dan SGPT Tikus.Skripsi. Fakultas Farmasi. Universitas Jember. 20219. [Online] Available: https://repository.unej.ac.id/handle/123456789/93593 [Accessed: Juli, 10, 2023].
Downloads
Additional Files
Posted
License
Copyright (c) 2023 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
