The Effect of Delay, Storing WholeBlood with K3EDTA and Na2EDTA Anticoagulants on Examination Results of Erythrocyte Counts, Hemoglobin Levels and Erythrocyte Morphology
Pengaruh Lama Waktu Penundaan dan Penyimpanan WholeBlood dengan Antikoagulan K3EDTA dan Na2EDTA Terhadap Hasil Pemeriksaan Jumlah Eritrosit, Kadar Hemoglobin dan Morfologi Eritrosit
DOI:
https://doi.org/10.21070/ups.8779Keywords:
K₃EDTA, Na₂EDTA, Erythrocyte Count, Hemoglobin, Erythrocyte MorphologyAbstract
Sample stability refers to the ability of blood to maintain its quantitative values under specific storage
conditions at room temperature and refrigerated temperature. This study aims to determine the differences in red blood cell count, hemoglobin levels, and erythrocyte morphology using K3EDTA vacutainer and conventional Na2EDTA blood samples
under varying temperatures and delay times. This experimental laboratory research used a cross-sectional
design involving four male students. Samples were stored at room temperature and cold temperature with delays of 0, 12, and 24 hours. Examinations were conducted using a hematology analyzer and blood smear preparations. Conclusion The type of anticoagulant and storage conditions did not significantly affect red blood cell count or hemoglobin levels. However, erythrocyte morphology showed alterations influenced by the type of EDTA, temperature, and storage duration. K₃EDTA was more stable in
maintaining erythrocyte morphology compared to Na₂EDTA, particularly at cold temperatures.
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