Analysis of LRGUK Gene Characters in Type II Diabetes Mellitus Patients in Sidoarjo
Analisis Karakter Gen LRGUK pada Penderita Diabetes Melitus Tipe II Di Sidoarjo
DOI:
https://doi.org/10.21070/ups.8771Keywords:
T2DM, LRGUK Gene, Sidoarjo, PolymorphismsAbstract
T2DM is a type diabetes mellitus that can be caused by bad habit or genetic factors, includinig the LRGUK gene, which has been analyzed before. However, the precise relationship between LRGUK gene and T2DM remains incompletely understood. This study aimed to analyze the characteristics of LRGUK gene in T2DM patients in Sidoarjo. Blood samples were collected from eight patients at Hospital of Bhayangkara Pusdik Shabara Porong, Sidoarjo. DNA was isolated and amplified using primers designed to targeting a 687 bp region of the LRGUK gene. Genetic analysis was performed using Mega X software which identified two points heterozygous AG mutation polymorphisms at 203 bp and 316 bp. The identified heterozygous mutation polymorphisms was not associated with T2DM. This conclusion supported by LRGUK GenBank data, which also shows different alleles (allele A and allele G) for nucleotide 203 bp and 316 bp.
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References
International Diabetes Federation, IDF Diabetes Atlas 2021 _ IDF Diabetes Atlas. 2021.
U. Galicia-Garcia et al., “Pathophysiology of Type 2 Diabetes Mellitus,” 2020. doi: 10.3390/ijms21176275.
E. Yunita et al., “Hubungan Pengaruh Polimorfisme Genetik terhadap Risiko Kejadian Diabetes Melitus,” Jurnal Medika Malahayati, vol. 6, no. 4, 2023, doi: 10.33024/jmm.v6i4.9004.
K. A. F. Imron et al., “Studi Kasus Penerapan Asuhan Keperawatan Gerontik pada Penderita Diabetes Melitus dengan Pendekatan Keluarga Binaan Di Desa Skardangan Sidoarjo,” IJoHVE: Indonesian Journal of Health Vovational Education, vol. 1, no. 2, pp. 56–66, Apr. 2022, doi: 10.36720/ijohve.v1i2.438.
N. Nina et al., “Determinan Risiko dan Pencegahan terhadap Kejadian Penyakit Diabetes Melitus Tipe 2 pada Usia Produktif di Wilayah DKI Jakarta,” Journal of Public Health Education, vol. 2, no. 4, 2023, doi: 10.53801/jphe.v2i4.148.
I. Barroso et al., “Candidate Gene Association Study in Type 2 Ddiabetes Indicates a Role for Genes Involved in β-cell Function as well as Insulin Action,” PLoS Biol, vol. 1, no. 1, 2003, doi: 10.1371/journal.pbio.0000020.
S. Kodama et al., “Quantitative Relationship between Cumulative Risk Alleles based on Genome-Wide Association Studies and Type 2 Diabetes Mellitus: A Systematic Review and Meta-analysis,” J Epidemiol, vol. 28, no. 1, 2018, doi: 10.2188/jea.JE20160151.
R. A. Salasa et al., “Faktor Risiko Diabetes Mellitus Tipe 2 pada Populasi Asia: A systematic Review,” Jurnal Biosaintek, vol. 1, no. 01, 2019, doi: 10.52046/biosainstek.v1i01.306.
A. R. Ishak et al., “Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity,” Hayati, vol. 21, no. 4, 2014, doi: 10.4308/hjb.21.4.159.
L. Resnyansky et al., “Reasons behind Reasons: A Communitarian Reading of Women’s Radicalization and Family Bombings in Southeast Asia,” Studies in Conflict and Terrorism, 2022, doi:
1080/1057610X.2022.2034229.
M. Mushlih et al., “Analisis Gen TCF7L2 (Trancription Factor 7 Like 2) pada Keluarga Penderita Diabetes Mellitus Tipe 2 Kecamatan Tanggulangin,” The Journal Of Muhammadiyah Medical Laboratory Technologist, vol. 3, no. 2, pp. 78–85, 2020.
W. O. A. H. Huesein and M. Mushlih, “Mutation Identification of rs7903146 Gene of Transcription Factor 7 Like 2 (TCF7L2) in Type II Diabetes Patients in Sidoarjo Region,” Unpublished, doi:
https://doi.org/10.21070/ups.2283.
H. S. Amin and M. Mushlih, “Identification of the Mitochondrial ND1 Gene Carrier of Diabetes Mellitus Type 2 with Blood Samples,” Medicra (Journal of Medical Laboratory Science/Technology), vol. 3, no. 2, pp. 48–53, Dec. 2020, doi: 10.21070/medicra.v3i2.873.
M. Mushlih et al., “Identification of Molecular Markers for Type 2 Diabetes Mellitus in Sidoarjo, Indonesia,” Jurnal Teknologi Laboratorium, vol. 9, no. 2, pp. 186–191, 2020, doi: 10.2938/teknolabjournal.v9i2.235.
E. W. Kartikasari and M. Mushlih, “Identification of Genes in Positive Alleles Marking Type II Diabetes Mellitus using Primer D20,” Indonesian Journal of Innovation Studies, vol. 15, Jul. 2021, doi: 10.21070/ijins.v15i.550.
M. Mushlih et al., “Specific Primer Design of Leucine Rich Repeats and Guanylate Kinase Domain Containing (LRGUK) Genes in Type II Diabetes Mellitus Patients,” Medical Technology and Public Health Journal, vol. 6, no. 2, pp. 195–200, Dec. 2022, doi: 10.33086/mtphj.v6i2.3476.
J. M. Laramie et al., “Polymorphisms Near EXOC4 and LRGUK on Chromosome 7q32 are Associated with Type 2 Diabetes and Fasting Glucose; The NHLBI Family Heart Study,” BMC Med Genet, vol. 9, May 2008, doi: 10.1186/1471-2350-9-46.
M. B. S. Al-Shuhaib and H. O. Hashim, “Mastering DNA Chromatogram Analysis in Sanger Sequencing for Reliable Clinical Analysis,” Journal of Genetic Engineering and Biotechnnology, vol. 21, no. 115, 2023, doi: 10.1186/s43141-023-00587-6.
C. N. Mahama and D. A. Suryandari, “Analisis Polimorfisme Gen dan Aplikasinya dalam Klinik,” Jurnal Biologi Papua, vol. 15, no. 1, pp. 88–98, April 2023, doi: 10.31957/jbp.2479.
H. Mustafa et al., “Variasi Genetik (Polimorfisme) Anopheles barbirostris dengan Menggunakan Metode PCR-RAPD,” in Prosiding Seminar Nasional Biologi FMIPA UNM Inovasi Penelitian Biologi dan Pembelajarannya di Era Merdeka Belajar, Makassar, 2020, pp. 97–104.
A. Susanti et al., “Peran Polimorfisme Gen GAS6 C.834 + 7AA pada Preeklamsia dan Normotensif di Kota Palembang,” Syntax Literate: Jurnal Ilmiah Indonesia, vol. 7, no. 6, pp. 8501–8515, Juni 2022, doi: 10.36418/syntax-literate.v7i6.8038.
M. R. Maftuha et al., “Urgensi Mempelajari Mutasi dan Variasi Genetik Berorientasi Content Representation untuk Memahami Evolusi Organisme,” Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi, vol. 8, no. 2, pp. 210–219, 2024, doi: 10.33369/diklabio.8.2.210-219.
J. Durland and H. Ahmadian-Moghadam, “Genetics, Mutagenesis,” in StatPearls (Internet), Treasure Island (FL): StatPearls Publishing, 2022. [Online]. Available: https://www.ncbi.nlm.nih.gov/books/NBK560519/
M. Khairani et al., “Literature Review: Perubahan Materi Genetik,” Jurnal Multidisiplin Ilmu Akademik, vol. 1, no. 3, pp. 872 – 879, Juni 2024, doi: 10.61722/jmia.v1i3.1803.
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