Comparison of Widal Test Result with Loop-Mediated Isothermal Amplification (LAMP) Test Result in Diagnosing Typhoid Fever
Perbandingan Hasil Pemeriksaan Widal dan Pemeriksaan Loop Mediated Isothermal Amplification (LAMP) dalam Menegakkan Diagnosa Demam Tifoid
DOI:
https://doi.org/10.21070/ups.8437Keywords:
Typhoid fever, Widal, LAMP, ExaminationAbstract
Typhoid fever is an infectious disease that is still common in developing countries, including Indonesia. Laboratory diagnosis often relies on the Widal test, which has limitations in detecting antibodies that can persist long after the infection has cleared, posing a risk of false positive or false negative results. As an alternative, the Loop-Mediated Isothermal Amplification (LAMP) method was developed to directly detect Salmonella typhi DNA with high sensitivity and specificity. The study design was descriptive analytical, involving 20 blood samples from patients at Aisyiyah Siti Fatimah General Hospital who had undergone the Widal test. The results showed 11 positive samples in LAMP and 9 negative samples. The Chi-square test yielded a p-value of 0.888 (p > 0.05), indicating no significant difference between the two methods. However, LAMP is superior in detecting active infections and could serve as a more accurate alternative method for diagnosing typhoid fever.
Downloads
References
M. Cut, “Pemeriksaan Laboratorium untuk Penunjang Diagnostik Demam Tifoid,” Jurnal Kesehatan Cehadum, vol. 1, no. 3, pp. 61–68, Sep. 2019.
N. Faruku et al., “Comparative analysis of widal agglutination and typhoid humoral response assays in febrile patients at Kebbi State, Nigeria,” Dutse Journal of Pure and Applied Sciences, vol. 10, no. 2a, pp. 313–323, Jul. 2024, doi: 10.4314/dujopas.v10i2a.30.
H. Nugroho, “Nilai Diagnostik Typhoid Dipstick Assay Pada Demam Tifoid,” Universitas Diponegoro Semarang, Semarang, 2016.
N. S. Mujahid et al., “Prevalence of typhoid fever among patients attending Murtala Muhammad Specialist Hospital Kano,” Bayero Journal of Pure and Applied Sciences, vol. 15, no. 1, pp. 57–63, Dec. 2022, doi: 10.4314/bajopas.v15i1.7.
I. Fathoni, “Hubungan Persinal Hygiene Dengan Kejadian Demam Tifoid Pada Santri,” Institusi Teknologi Sains Dan Kesehatan Insan Cendekia Medika Jombang, Jombang, 2024. [Online]. Available: www.jpns-journal.com
S. Surya Brilianto, A. Mulyanto, R. Sulistiyowati, and M. Rahaju, “Perbandingan Hasil Pemeriksaan Demam Tifoid Menggunakan Metode Widal Slide Dan Metode Rapid Test IgG IgM Di RSUD Dr R Goeteng Taroenadibrata,” Jurnal Ilmiah Multidisiplin, vol. 3, no. 10, 2024.
S. Jeevan, “Use of widal test in diagnosis of typhoid fever in North Indian population by estimating baseline titer in control group,” Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 7, pp. 251–253, 2017, doi: 10.22159/ajpcr.2017.v10i7.18789.
A. Rahayu, V. Krisdianilo, S. Hutabarat, adah Siregar, and V. Ade Rizky, “Evaluasi Hasil Titer Pada Pemeriksaan Widal Berdasarkan Lama Demam Di Rumah Sakit Grandmed Lubuk Pakam,” Jurnal Farmasi, vol. 4, no. 2, pp. 2655–0814, Nov. 2022, Accessed: May 05, 2025. [Online]. Available: http://ejournal.medistra.ac.id/index.php/JFM
P. Prakash, K. Rajpal, and S. Mahto, “Comparative evaluation of diagnostic efficacy of widal slide agglutination test & widal tube agglutination test in enteric fever,” International Journal of Medical and Health Research Original Research Article International Journal of Medical and Health Researchmedicalsciencejournal.com, vol. 4, pp. 181–183, 2018, [Online]. Available: www.medicalsciencejournal.com
A. Fatmawati Rachman, “Uji Diagnostik Tes Serologi Widal Dibandingkan Dengan Kultur Darah Sebagai Baku Emas Untuk Diagnosis DemamTifoid Pada Anak Di RSUP Dr. Kariadi Semarang,” Semarang, p. 1, 2011. Accessed: May 23, 2025. [Online]. Available: https://eprints.undip.ac.id/32794/
A. L. Olopoenia and A. King L, “Widal agglutination test − 100 years later:still plagued by controversy,” Postgrad Med F, vol. 76, pp. 80–84, 2020, Accessed: May 05, 2025. [Online]. Available: https://pubmed.ncbi.nlm.nih.gov/10644383/
S. Putri Ramadhanti, “Optimasi Metode Analisis Daging Babi Berbasis DNA Menggunakan Loop-Mediated Isothermal Amplification,” Universitas Brawijaya, Malang, 2021.
O. S. Ningsih, A. I. R. Detha, and D. A. Wuri, “Studi Literatur Metode Diagnosis Anisakiasis,” Jurnal Veteriner Nusantara, vol. 6, no. 1, pp. 1–23, 2023, doi: 10.35508/jvn.v6i1.3173.
Murwantoko, “Metode Loop- Mediated Isothermal Amplification (LAMP) Dan Aplikasinya Untuk Deteksi Penyakit Ikan,” Jurnal Perikanan, vol. 8, no. 1, pp. 1–8, 2006, [Online]. Available: www.pubmed.
S. Sumathi, D. Venkatesh, and S. Krishna, “A Comparative Study Of Typhidot And Widal Test In The Diagnosis Of Typhidot Fever,” 2013.
D. Sari, A. Tantri, and lisna Dewi, “Imunuserologi Test Widal,” Politeknik Kesehatan Denpasar, Denpasar, 2020. Accessed: May 08, 2025. [Online]. Available: https://www.scribd.com/document/444956104/Pemeriksaan-widal-klp4-4A-docx?utm_source=chatgpt.com
G. Ramaningrum, H. D. Anggraheny, and T. P. Putri, “Faktor-faktor yang Mempengaruhi Kejadian Demam Tifoid pada Anak di RSUD Tugurejo Semarang,” Semarang, pp. 1–8. Accessed: May 18, 2025. [Online]. Available: https://jurnal.unimus.ac.id/index.php/kedokteran/article/viewFile/2596/2445
H. Ou, Y. Wang, J. Gao, J. Bai, Q. Zhang, and L. Shi, “Rapid detection of salmonella based on loop-mediated isothermal amplification,” Ann Palliat Med, vol. 10, no. 6, pp. 6850–6858, Jun. 2021, doi: 10.21037/apm-21-1387.
D. Hardianto, “Telaah Metode Diagnosis Cepat dan Pengobatan Infeksi Salmonella typhi,” Jurnal Bioteknologi & Biosains Indonesia, vol. 6, no. 1, pp. 149–158, Jun. 2019, doi: 10.29122/jbbi.v6i1.2935.
C. Putri Melinia, “Hubungan Titer Antibodi Uji Widal Dengan Hitung Neutrofil Dan Limfosit Pasien Demam Tifoid Di Salah Satu RS Swasta Bintaro,” STIkes Mitra Keluarga, Bekasi, 2021. Accessed: Jun. 03, 2025. [Online]. Available: https://repository.stikesmitrakeluarga.ac.id/repository/CINDANI%20MELINIA%20PUTRI_201803010_KTI%20TLM_IMUNOSEROLOGI_2021.pdf
M. Soroka, B. Wasowicz, and A. Rymaszewska, “Loop-mediated isothermal amplification (Lamp): The better sibling of pcr?,” Aug. 01, 2021, MDPI. doi: 10.3390/cells10081931.
A. M. Badri, H. M. Ahmed, N. Y. Ahmed, H. H. Ahmed, S. M. Ali, and S. M. Sid, “A Comparison Study of Serological Test, Culturing Technique and Molecular Detection of Salmonella Typhi Among Febrile Patients in Khartoum State, Sudan,” Advances in Biotechnology & Microbiology, vol. 13, no. 4, Mar. 2019, doi: 10.19080/aibm.2019.13.555866.
Downloads
Additional Files
Posted
License
Copyright (c) 2025 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
