The detection of Toxoplasma gondii in cat using the Microscopic and Loop-Mediated Isothermal Amplification (LAMP) method at the Larangan Market Sidoarjo
Deteksi Toxoplasma gondii Pada Feses Kucing Menggunakan Metode Mikroskopis dan Metode Loop-Mediated Ishotermal Amplification (LAMP) di Pasar Larangan Sidoarjo
DOI:
https://doi.org/10.21070/ups.7747Keywords:
Toxoplasmosis, toxoplasma gondii, saturated NaCl Flotation, LAMPAbstract
Toxoplasmosis, caused by Toxoplasma gondii, is a zoonotic disease affecting both humans and animals, particularly those with weakened immune systems. This study aimed to detect T. gondii in cat feces using saturated NaCl microscopy and the Loop-Mediated Isothermal Amplification (LAMP) method, while also evaluating the sensitivity of LAMP. Samples were collected using accidental sampling over one month in August. A descriptive analysis compared microscopy and LAMP results using the SPSS version 23 kappa test. Of the 9 samples from Larangan Market, Sidoarjo, 4 tested positive and 5 negative. Detection involved NaCl flotation, DNA isolation via the resin method, and LAMP with visual color indicators. Sensitivity testing used fivefold PBS dilution to improve DNA quality. The results showed perfect agreement between methods (k = 1.000, p < 0.05), indicating high reliability. Further studies should increase sample size and diversity to strengthen population representation and result validity.
Downloads
References
S. Budi, G. Abimanyu, and D. Sulistyawati, “Proceeding 2 nd Setiabudi-Cihams 2022 Prevalensi dan Faktor Risiko Penularan Toxoplasmosis pada Pemotong dan Penggiling Daging di RPH Jagalan Surakarta Prevalence and Risk Factors for The Transmission of Toxoplasmosis in Meat Cutters and Meat Grinder in Jagalan Slaughterhouse Surakarta,” 2022.
J. M. Mose, J. M. Kagira, D. M. Kamau, N. W. Maina, M. Ngotho, and S. M. Karanja, “A Review on the Present Advances on Studies of Toxoplasmosis in Eastern Africa,” 2020, Hindawi Limited. doi: 10.1155/2020/7135268.
A. A. Aguirre et al., “The One Health Approach to Toxoplasmosis: Epidemiology, Control, and Prevention Strategies,” Jun. 15, 2019, Springer New York LLC. doi: 10.1007/s10393-019-01405-7.
I. Rahman and A. Nur, “Identifikasi Toxoplasma gondii Terhadap Feses Kucing Peliharaan Sebagai Sumber Penyebaran Toxoplasmosis di Kota Ternate,” SAINTIFIK, vol. 8, no. 2, pp. 146–150, Jul. 2022, doi: 10.31605/saintifik.v8i2.353.
E. S. Al-Malki, “Toxoplasmosis: stages of the protozoan life cycle and risk assessment in humans and animals for an enhanced awareness and an improved socio-economic status,” Jan. 01, 2021, Elsevier B.V. doi: 10.1016/j.sjbs.2020.11.007.
P. R. Torgerson and P. Mastroiacovo, “La charge mondiale de la toxoplasmose: une étude systématique,” Bull World Health Organ, vol. 91, no. 7, pp. 501–508, Jul. 2013, doi: 10.2471/BLT.12.111732.
A. Retmanasari, B. S. Widartono, M. A. Wijayanti, and W. T. Artama, “Prevalence and Risk Factors for Toxoplasmosis in Middle Java, Indonesia,” Ecohealth, vol. 14, no. 1, pp. 162–170, Mar. 2017, doi: 10.1007/s10393-016-1198-5.
R. Zakaria and S. Ardiansyah, “Potential Analysis Of Toxoplasmosis Distribution In Wild Cats (Felis silvestris) In Some Markets Of Sidoarjo District Through Microscopic Identification Of Toxoplasma gondii,” Medicra (Journal of Medical Laboratory Science/Technology), vol. 3, no. 2, pp. 59–64, Dec. 2020, doi: 10.21070/medicra.v3i2.890.
K. Brebes et al., “Hubungan Antara Faktor Risiko Demografi Terhadap Kejadian Toksoplasmosis Pada Masyarakat di Kabupaten Brebes dan Kabupaten Kendal Jawa Tengah,” 2017.
H. Mirahmadi, R. Hasanzadeh, H. M. Raeesi, S. Fallahi, K. Shahraki, and A. Badirzadeh, “Loop-Mediated Isothermal Amplification (LAMP) Assay to De-tect Toxoplasmosis in Schizophrenia Patients,” 2020. [Online]. Available: http://ijpa.tums.ac.ir
R. Augustine et al., “Loop-mediated isothermal amplification (Lamp): A rapid, sensitive, specific, and cost-effective point-of-care test for coronaviruses in the context of covid-19 pandemic,” Aug. 01, 2020, MDPI AG. doi: 10.3390/biology9080182.
T. Notomi, Y. Mori, N. Tomita, and H. Kanda, “Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects,” Jan. 17, 2015, Microbiological Society of Korea. doi: 10.1007/s12275-015-4656-9.
L. Sheng et al., “Rapid and visual detection of Toxoplasma gondii oocyst in cat feces using loop-mediated isothermal amplification (LAMP) assay,” Sci Rep, vol. 13, no. 1, Dec. 2023, doi: 10.1038/s41598-023-44658-7.
L. D. Jayanti and M. Mushlih, “Comparison of the Quality of DNA Template Isolation Results of the Resin Method with and Without Centrifugation,” Indonesian Journal of Innovation Studies, vol. 15, Jul. 2021, doi: 10.21070/ijins.v15i.551.
A. A. Setiaputri et al., “Perbandingan Metode Isolasi DNA pada Produk Perikanan Segar dan Olahan,” 2020.
J. W. Park, “Principles and Applications of Loop-Mediated Isothermal Amplification to Point-of-Care Tests,” Oct. 01, 2022, MDPI. doi: 10.3390/bios12100857.
W. N. Rahmah, F. H. Ramdhani, and A. Hidayani, “Gambaran Hasil Uji Sensitivitas Antibiotik Terhadap Bakteri Escherichia coli dengan Metode DISC dan Sumuran,” Jurnal Surya Medika, vol. 10, no. 2, pp. 344–348, Aug. 2024, doi: 10.33084/jsm.v10i2.7495.
Downloads
Additional Files
Posted
License
Copyright (c) 2025 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
