Isolation and Identification of Fungal Contaminants in Porang (Amorphopallus muelleri blume) Plant Tissue Culture with the Addition of Hormone BAP (Benzyl Amino Purine)
Isolasi dan Identifikasi Kontaminan Jamur pada Kultur Jaringan Tanaman Porang (Amorphopallus muelleri blume) dengan Penambahan Hormon BAP (Benzyl Amino Purine)
DOI:
https://doi.org/10.21070/ups.7323Keywords:
Porang plant, Tissue culture, Contaminant fungi, BAP (Benzyl Amino Purine)Abstract
This research identifies and describes contaminant fungi in Porang (Amorphophallus muelleri Blume) Madiun variety planlets, providing insights into their characteristics as a basis for contamination control. This is crucial for enhancing Porang cultivation, productivity, and seedling quality. Conducted from August to November 2024 at the Tissue Culture Laboratory of CV. Embryo Multi Agro and the Microbiology and Biotechnology Laboratory of Muhammadiyah Sidoarjo University, the study used a descriptive approach. Planlets in the shoot growth phase were cultured in 1.5 ppm BAP (Benzyl Amino Purine) media. The study examined colony color, growth direction, surface shape, hyphae structure, and spore or conidia production. Five contaminant fungi were identified: Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus, Rhizoctonia solani, and Rhizopus sp. These findings provide essential data for contamination management in Porang tissue culture.
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References
M. Nur Khozin, D. Pudji Restanto, and D. Erwin Kusbianto, “Somatik Embriogenesis Langsung dan Tidak Langsung pada Tanaman Porang (Amarphopallus oncophilus),” vol. 2, no. 7, pp. 42–45, 2022, doi: 10.33661/jai.v7i2.6053.
Z. I. Lailani and P. C. Kuswandi, “Pengaruh Penambahan BAP Terhadap Induksi Kalus Tanaman Porang Secara In Vitro,” Jurnal Kingdom The Journal of Biological Studies, vol. 9, no. 1, pp. 45–55, 2023, [Online]. Available: https://journal.student.uny.ac.id/
KBRI Tokyo, Laporan Analisis Intelijen Bisnis Porang. Tokyo: Atase Perdagangan KBRI Tokyo, 2021.
T. N. Khoiriah, Z. Nuraini, K. Novia Andriani, D. Wulandariningtyas, F. Wirayudha, and Wuryantoro, “Kajian Tingkat Kontaminasi Pada Kultur Jaringan Tanaman Porang,” Agri-Tek: Jurnal Penelitian Ilmu Ilmu Esakta, vol. 23, pp. 20–24, 2022, [Online]. Available: http://agritek.unmermadiun.ac.id/index.php/agritek
Haerunnisa, Fitri Hayati, and Ricky Firmansyah, “Analisis Potensi dan Pemetaan Tanaman Porang pada Pasar Ekspor Internasional,” Jurnal Manajemen Bisnis, Akuntansi dan Keuangan, vol. 2, no. 1, pp. 15–26, 2023, doi: 10.55927/jambak.v2i1.4209.
Khoirunnisa and I. S. Mercuriani, “Optimasi Teknik Sterilisasi Eksplan dan Medium Induksi Kalus Porang (Amorphophallus muelleri Blume) dengan Penambahan Zat Pengatur Tumbuh (ZPT) 2,4-D,” 2022. [Online]. Available: https://journal.student.uny.ac.id/
S. Pancaningtyas, N. Afni, and H. Dewi, “Pengembangan Porang (Amorphopalus muelleri) Melalui Teknik Perbanyakan Kultur Jaringan sebagai Tanaman Sela Komoditas Perkebunan,” Warta: Pusat Penelitian Kopi dan Kakao Indonesia, 2021.
A. Adawiyah et al., “Optimasi Sterilisasi Eksplan Umbi dan Bulbil Porang (Amorphopalus Muelleri Blume.) pada Kultur In Vitro,” Agroscript, vol. 3, no. 2, pp. 121–131, 2021.
V. Yuliarbi Farlisa, P. Dewanti, K. Hariyono, T. Handoyo, and D. Pudji Restanto, “Induksi Somatic Embriogenesis dan Kultur Suspensi Sel pada Tanaman Porang (Amorphophallus muelleri Blume) Induction of Somatic Embryogenesis and Cell Suspension Culture in Amorphophallus muelleri Blume,” Agriprima Journal of Applied Agricultural, 2022, doi: 10.25047/agriprima.v6i1.448.
E. Lailatul Maghfiroh, A. Munif, A. A. Nawangsih, and A. Akhdiya, “Karakterisasi Bakteri Penyebab Busuk Lunak pada Umbi Porang (Amorphophallus muelleri) Menggunakan Primer PCR Spesifik,” Jurnal Ilmu Pertanian Indonesia, vol. 27, no. 3, pp. 463–472, Jul. 2022, doi: 10.18343/jipi.27.3.463.
U. D. Citra, R. N. M. Ridwan, P. Indriani, and U. Haniyah, “Etika dalam Penanganan Eksplan Kultur Jaringan Pisang Barangan (Musa Acuminata L.) yang Terkontaminasi Perspektif Keamanan Pangan dan Kesehatan Masyarakat,” Jurnal Pendidikan Biologi, pp. 1148–1154, 2024.
I. Alfiana, “Pengaruh Kombinasi Zat Pengatur Tumbuh Air Kelapa, BAP dan NAA pada Media DKW Terhadap Pertumbuhan Eksplan Rumput Gajah (Pennisetum purpureum schumach) Secara In Vitro,” Universitas Siliwangi, no. Skripsi, 2020.
D. N. Hariyanto, T. Handoyo, P. Dewanti, K. Hariyono, and D. P. Restanto, “Analisis Histologi dan Scaning Elektron Mikroskopy (SEM) pada Somatik Embriogenesis Tanaman Porang (Amorphophallus muelleri B),” Agriprima : Journal of Applied Agricultural Sciences, vol. 6, no. 1, pp. 22–34, 2022, doi: 10.25047/agriprima.v6i1.450.
F. C. Haq, Maftuchah, and A. Zainudin, “Effect Of Indole Butyric Acid (Iba) and Thidiazuron (Tdz) Concentrations on Explant Growth Planlet Porang (Amorphophallus Muelleri Blume.) In Vitro Culture,” Journal of Tropical Crop Science and Technology, vol. 4, no. 2, pp. 133–145, Oct. 2022, doi: 10.22219/jtcst.v4i2.32484.
Andriani C, Fadhila F. R., and Kodariah L., “Identification ff Aspergillus sp Growth on White Bread Against Storage Temperature,” vol. 10, no. 2, 2020.
D. Andriani and P. Heriansyah, “Identifikasi Jamur Kontaminan pada Berbagai Eksplan Kultur Jaringan Anggrek Alam (Bromheadia finlaysoniana (Lind.) Miq,” Agro Bali : Agricultural Journal, vol. 4, no. 2, pp. 192–199, 2021, doi: 10.37637/ab.v4i2.723.
I. E. Girsang, R. Restiani, and D. A. Prasetyaningsih, “Callus Induction of Porang (Amorphophallus mueleri Blume) Leaf Explant Using Combination of Coconut Water and IAA (Indole Acetic Acid),” Yogyakarta, 2023.
W. Yali, “Plant Tissue Culture and its Application in Modern Crop Improvement,” International Research Journal of Plant and Crop Sciences (IRJPCS), vol. 6, pp. 201–0, 2021, [Online]. Available: www.advancedscholarsjournals.org
E. D. Sulichantini, A. P. D. Nazari, and A. Nuanyah, “Identifikasi Kontaminasi Kultur Jaringan Pisang Cavendish,” Jurnal Agrotek Tropika, vol. 12, no. 2, pp. 400–409, 2024, doi: 10.23960/jat.v12i2.6721.
A. Rosyidi Abdullah, A. Nurokhman, S. Citra Rahayu, E. Metalisa, and L. Novitasari, “Faktor Kontaminasi Kultur Jaringan pada Eksplan Biji Duku (Lansium Domesticum Corr.) Menggunakan Media Murashige And Skoog,” Prosiding Seminar Nasional Pendidikan Biologi, pp. 146–131, 2022, [Online]. Available: http://proceedings.radenfatah.ac.id/index.php/semnaspbio
B. Herdiawan, “Produksi Benih Kentang (Solanum tuberosum L.) Secara In Vitro dI CV Embrio Multi Agro,” Institur Pertanian Bogor, 2023.
A. I. Sucahyo, K. Manalu, and R. A. Nasution, “Isolasi dan Identifikasi Mikroba Penyebab Kontaminasi dari Udara di Laboratorium Kultur Jaringan Tumbuhan UIN-SU Medan,” J Biol (Denpasar), vol. 1, no. 1, pp. 1–12, 2023, doi: 10.47134/biology.v1i1.1931.
M. Nur Khozin and D. Pudji Restanto, “Regenerasi Tanaman Porang (Amarphopalus Onchopillus) Secara In Vitro Dengan Eksplan Daun,” Agritrop: Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), vol. 20, no. 1, pp. 59–77, 2022, [Online]. Available: http://jurnal.unmuhjember.ac.id/index.php/AGRITROP
Y. Suryani, O. Taupiqurrahman, and Y. Kulsum, Buku Mikologi, 1st ed., vol. 1. Padang: PT. Freeline Cipta Granesia, 2020.
P. Helena, “Identifikasi Jamur Mikroskopis Pembusuk Buah-Buahan dalam Bentuk Preparat Sebagai Bahan Ajar Mikologi,” Jambi, 2022.
R. Odrina, “Isolasi Dan Identifikasi Jamur Mikroskopis pada Gula Aren Hasil Produksi Masyarakat Maro Sebo Sebagai Bahan Ajar Mikologi dalam Bentuk Buku Saku,” Jambi, 2023.
H. L. Barnett and B. B. Hunter, “Illustrated Genera of Imperfect Fungi,” 1987.
M. I. Mahendra, M. Martosudiro, and F. A. Choliq, “Eksplorasi Jamur Tanah yang Berpotensi sebagai Bioremediator Fungisida Berbahan Aktif Propineb pada Tanaman Jeruk (Citrus reticulata L.),” Jurnal Hama dan Penyakit Tumbuhan, vol. 10, no. 4, pp. 174–186, 2022, doi: 10.21776/ub.jurnalhpt.2022.010.4.3.
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