The Effectiveness of Castor Bean Leaves (Ricinus communis L.) in Reducing Lead (Pb) Levels in Wistar Rats (Rattus norvegicus) In Vivo
Efektivitas Ekstrak Daun Jarak Kepyar (Ricinus communis L.) terhadap Penurunan Kadar Logam Berat Timbal (Pb) pada Tikus Wistar (Rattus norvegicus) secara In Vivo
DOI:
https://doi.org/10.21070/2cx0hm59Keywords:
Ricinus communis L., Pb, Rats, Atomic Absorption Spectrophotometry (AAS)Abstract
Pb is a heavy metal that accumulates in the body and causes health problems. This study investigated castor bean leaf extract (Ricinus communis L.) for reducing blood Pb levels in Wistar rats (Rattus norvegicus). Leaves from Jagir and Krian were combined after drying, while separate phytochemical screenings showed identical results. This laboratory experiment used a pretest-posttest control group design involving 24 male rats divided into six groups: negative, positive, 0.5% Na-CMC controls, and extract doses of 250, 500, and 750 mg/kg body weight. Rats received lead acetate (700 mg/kg) for seven days, followed by extract treatment for 14 days. Blood Pb was measured using AAS at 217 nm. All groups showed decreases. Extract groups decreased by 0.1442, 0.2221, and 0.3224 mg/L, respectively. The greatest decrease occurred at 750 mg/kg, from 0.4307 to 0.1083 mg/L. Wilcoxon testing showed no significant difference (p=0.068). The extract showed potential to reduce blood Pb levels.
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