Analysis of Differences in Serum Electrolyte Levels Between the Direct ISE and Indirect ISE Methods in Patients with Coronary Heart Disease
Analisis Perbedaan Hasil Elektrolit Serum Metode Direct ISE dengan Indirect ISE pada Pasien Jantung Koroner
DOI:
https://doi.org/10.21070/ups.11372Keywords:
Coronary Heart, Serum, Direct ISE, Indirect ISE, Sodium, potassium, ChlorideAbstract
Coronary heart disease (CHD) is increasing in Indonesia, and electrolyte imbalances involving sodium, potassium, and chloride may contribute to its occurrence. Serum electrolyte testing is performed using Ion-Selective Electrode (ISE) technology through direct and indirect methods. This study evaluated the agreement between these methods in CHD patients. An experimental study was conducted at the Central Laboratory of Dr. Saiful Anwar Regional General Hospital, East Java, using 50 serum samples. Electrolyte levels were measured with the Rapidchem 744 Analyzer (direct ISE) and Cobas Pro (indirect ISE). Statistical analysis included the paired-sample t-test for sodium and chloride and the Wilcoxon signed-rank test for potassium. No significant differences were found between the two methods for sodium and chloride (p = 1.000 and p = 0.125). Potassium showed a significant difference (p < 0.001); however, the mean difference remained within the Clinical Laboratory Improvement Amendments (CLIA) tolerance limit of ±0.3 mmol/L.
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