Identification of Ascaris lumbricoides Infection in the Stool Samples of Students at the Preschool in Nangahure Lembah Village, Using the Formol-Ether Sedimentation Method and the Loop-Mediated Isothermal Amplification (LAMP) Method.
Identifikasi Infeksi Ascaris lumbricoides pada Feses Siswa Pra-sekolah di Desa Nangahure Lembah Menggunakan Metode Sedimentasi Formol-Ether dan Metode Loop Mediated Isothermal Amplification (LAMP)
DOI:
https://doi.org/10.21070/ups.11358Keywords:
Ascariasis, Ascaris lumbricoides, Formol-Ether Sedimentation, LAMPAbstract
Ascariasis is a disease caused by a soil-transmitted helminth infection specifically, the parasite Ascaris lumbricoides that affects health, particularly in preschool-aged children with compromised immune systems. This study aimed to detect A. lumbricoides in the stool samples of preschoolers using the formalin-ether sedimentation microscopy method and the Loop-Mediated Isothermal Amplification (LAMP). Samples were collected using the total sampling technique over a two-week period in April. Of the 30 stool samples analyzed, the microscopic method identified 24 positive and 6 negative samples for A. lumbricoides; some of these samples were co-infected or triple-infected with Strongyloides, Schistosoma, Trichuris trichiura, Toxoplasma, and Taenia sp. The LAMP method identified 26 positive and 4 negative samples. In a concordance test using SPSS with the Kappa test, the results showed near perfect agreement with a value of (k=0.762) and (p<0.05).
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