Detection of Dengue Virus Using the Loop-Mediated Isothermal Amplification (LAMP) Method in Suspected Dengue Fever Patients Based on Platelet Count
Deteksi Virus Dengue Menggunakan Metode Loop-Mediated Isothermal Amplification (LAMP) pada Pasien Terduga Demam Berdarah Berdasarkan Nilai Trombosit
DOI:
https://doi.org/10.21070/ups.11357Keywords:
Dengue Hemorrhagic Fever, thrombocytopenia, RT-LAMP, dengue virus, dengue viral RNAAbstract
Dengue Hemorrhagic Fever (DHF) remains a major public health problem in Indonesia. Thrombocytopenia is a laboratory finding commonly used as an indicator for the diagnosis and monitoring of patients with DHF. Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) is a molecular diagnostic method used to detect dengue virus RNA. This laboratory experimental study aimed to determine the relationship between thrombocytopenia categories and RT-LAMP results in 30 patients suspected of having DHF based on the physician’s provisional diagnosis. Platelet counts were measured using a Zybio hematology analyzer and categorized into mild, moderate, severe, and very severe thrombocytopenia. RT-LAMP targeting the C-prM gene detected dengue virus RNA in 26 samples (86.7%), while 4 samples (13.3%) tested negative. Statistical analysis using the Chi-Square test with Fisher’s Exact Test interpretation showed no significant association between thrombocytopenia categories and RT-LAMP results (p = 0.696). Positive RT-LAMP results were observed across all thrombocytopenia categories.
Downloads
References
D. A. Ira, “Gambaran Penyakit dan Vektor Demam Berdarah Dengue (DBD) Perguruan Tinggi di Medan, Sumatera Utara,” J. War., vol. 48, no. 1, pp. 1–15, 2016.
F. Nugraha, B. Haryanto, R. A. Wulandari, and T. T. Pakasi, “Studi Ekologi Hubungan Kejadian Demam Berdarah Dengue (DBD) dengan Faktor Iklim di Kota Administrasi Jakarta Pusat, Indonesia Tahun 1999-2018,” J. Ilmu Kesehat., vol. 10, no. 01, pp. 93–112, 2021, doi: https://doi.org/10.33221/jikm.v10i03.923.
World Health Organization, “Disease Outbreak News: Dengue - Global situation,” World Health Organization. Accessed: Jan. 10, 2026. [Online]. Available: https://www.who.int/emergencies/disease-outbreak-news/item/2024-DON518?utm
Kementerian Kesehatan RI., “Waspada Penyakit di Musim Hujan,” Kementerian Kesehatan RI. Accessed: Jan. 10, 2026. [Online]. Available: https://kemkes.go.id/id/waspada-penyakit-di-musim-hujan
Badan Pusat Statistik Provinsi Jawa Timur, “Kasus Penyakit Menurut Kabupaten/Kota dan Jenis Penyakit di Provinsi Jawa Timur, 2025,” Badan Pusat Statistik Provinsi Jawa Timur. Accessed: Apr. 12, 2026. [Online]. Available: https://jatim.bps.go.id/id/statistics-table/3/YTA1Q1ptRmhUMEpXWTBsQmQyZzBjVzgwUzB4aVp6MDkjMw==/kasus-penyakit-menurut-kabupaten-kota-dan-jenis-penyakit-di-provinsi-jawa-timur--2024.html
A. Murugesan and M. Manoharan, “Dengue Virus,” in Emerging and Reemerging Viral Pathogens, Emerging and Reemerging Viral Pathogens, 2020, pp. 281–358. doi: https://doi.org/10.1016/B978-0-12-819400-3.00016-8.
World Health Organization, “Dengue,” World Health Organization. Accessed: Apr. 12, 2026. [Online]. Available: https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue
R. Halim and M. Rifal, “Trombositopenia pada Demam Berdarah Dengue,” UMI Med. J., vol. 9, no. 1, pp. 1–9, 2024, doi: 10.33096/umj.v9i1.288.
I. P. Sari, Y. Rahmawati, and W. S. Hadi, “Analisis Hasil Quality Control Pemeriksaan Trombosit Dan Hemogoblin Di RSUD Wates Yogyakarta,” J. Kesehat. TAMBUSAI, vol. 6, no. 4, pp. 17998–18007, 2025.
V. Chakradhar, R. Kashyap, J. Christopher, and A. Bekele, “Thrombocytopenia in adult patients with sepsis : incidence , risk factors , and its association with clinical outcome,” J. Intensive Care, vol. 1, no. 1, pp. 1–10, 2013, doi: 10.1186/2052-0492-1-9.
N. P. D. W. N. W. E. Yanti, I. G. A. M. Putri, I. W. G. Suarjana, “Hubungan Antara Uji Antigen Non Structural 1 (NS1) Dengan Kejadian Trombositopenia Pada Kasus Demam Dengue (DD)/Demam Berdarah Dengue (DBD) di Rumah Sakit Ari Canti, Gianyar, Bali Tahun 2016,” J. Med. Udayana, vol. 9, no. 6, pp. 79–83, 2020.
C. T. S. I. M. S. Utama, S. Lukman, S. H. Sukabdi, P. H. Sinto, “Dengue Viral Infection in Indonesia: Epidemiology, Diagnostic Challenges, and Mutations from an Observational Cohort Study,” BMC Infect. Dis., vol. 19, no. 1, pp. 1–19, 2019.
L. Meilany, A. S. Sukawan, and N. Fadillah, “Hubungan Pengetahuan dan Kesesuaian Pemeriksaan Klinis dengan Ketepatan Kode Diagnosa Demam Berdarah Dengue di RSKD Ibu dan Anak Siti Fatimah,” J. Manaj. Inf. Kesehat. Indones., vol. 8, no. 2, pp. 143–147, 2020, doi: 10.33560/jmiki.v8i2.282.
P. Hamid, A. Widyasari, and A. Krediastuti, “Application of Combined Multiplex-PCR and RT-LAMP to Detect Dengue Fever, from Clinically Infected Patients in Surabaya, Indonesia,” Int. Res. J. Microbiol., vol. 6, no. 1, pp. 1–5, 2015, doi: http:/dx.doi.org/10.14303/irjm.2014.040.
J. W. M. F. Wowor, S. R. Umboh, “Deteksi Dini Demam Berdarah Dengue dengan Pemeriksaan Antigen NS1,” e-Clinic, vol. 5, no. 2, pp. 1–9, 2017.
G. C. W. K. Tan, H. L. C. Chu, S. Shrivastava, J. Gunaratna, K. S. Tan, “Operational utility of the reverse- transcription recombinase polymerase amplification for detection of dengue virus,” BMC Infect. Dis., vol. 18, no. 1, pp. 1–9, 2018, doi: 10.1186/s12879-018-3074-9.
R. V. B. M. D. A. L. Dauner, I. R. G. S. Mesquita, L. M. S. Santos, M. G. S. Silva, “Development of a Pan-Serotype Reverse Transcription Loop-Mediated Isothermal Amplification Assay for the Detection of Dengue Virus”,” Diagn. Microbiol. Infect. Dis., vol. 83, no. 1, pp. 30–36, 2015, doi: 10.1016/j.diagmicrobio.2015.05.004.
K. M. M. Parida, G. Posadas, S. Inoue, F. Hasebe, “Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Assay,” J. Clin. Microbiol., vol. 43, no. 6, pp. 2895–2903, 2005, doi: 10.1128/JCM.43.6.2895-2903.2005.
B. T. Teoh, S. S. Sam, and K. K. Tan, “Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification,” BMC Infect. Dis., vol. 13, no. 1, p. 1, 2013, doi: 10.1186/1471-2334-13-387.
A. K. Sahni, N. Grover, A. Sharma, I. D. Khan, and J. Kishore, “Reverse transcription loop-mediated isothermal amplification ( RT-LAMP ) for diagnosis of dengue,” MJAFI, vol. 69, no. 3, pp. 246–253, 2013, doi: 10.1016/j.mjafi.2012.07.017.
L. Guo, Y. Gu, and Y. Zhang, “Platelet Dynamics and Thrombocytopenia in Dengue Fever: A Prospective Cohort Study from Shenzhen, China,” New Microbes New Infect., vol. 67, p. 101624, 2025, doi: 10.1016/j.nmni.2025.101624.
R. M. Y. L. Lau, M. Y. Lai, B. T. Teoh, S. S. Sam, S. AbuBakar, “Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification,” PLoS One, vol. 10, no. 9, pp. 1–9, 2015, doi: 10.1371/journal.pone.0138694.
R. J. Sapia, C. Campbell, A. J. Reed, V. B. Tsvetkov, and Y. V. Gerasimova, “Dyes and Pigments Interaction of GelRed TM with single-stranded DNA oligonucleotides : Preferential binding to thymine-rich sequences,” Dye. Pigment., vol. 188, no. 109209, pp. 1–7, 2021, doi: 10.1016/j.dyepig.2021.109209.
E. L. de Azeredo, R. Q. Monteiro, and L. M. de-O. Pinto, “Thrombocytopenia in dengue: Interrelationship between virus and the imbalance between coagulation and fibrinolysis and inflammatory mediators,” Mediators Inflamm., vol. 2015, no. 313842, pp. 1–16, 2015, doi: 10.1155/2015/313842.
B. E. E. Martina, P. Koraka, and A. D. M. E. Osterhaus, “Dengue Virus Pathogenesis : an Integrated View,” Clin. Microbiol. Rev., vol. 22, no. 4, pp. 564–581, 2009, doi: 10.1128/CMR.00035-09.
V. F. La Russa and B. L. Innis, “Mechanisms of dengue virus-induced bone marrow suppression.,” Baillieres. Clin. Haematol., vol. 8, no. 1, pp. 249–270, Mar. 1995, doi: 10.1016/s0950-3536(05)80240-9.
F. I. A. S. Khazali, W. H. Hadrawi, “Thrombocytopenia in Dengue Infection: Mechanisms and a Potential Application,” Expert Rev. Mol. Med., vol. 26, no. e67, pp. 1–15, 2024, doi: 10.1017/erm.2024.18.
N. L. Vuong, N. T. H. Quyen, N. T. H. Tien, and K. Duong Thi Hue, “Dengue viremia kinetics and effects on platelet count and clinical outcomes: An analysis of 2340 patients from Vietnam,” Elife, vol. 13, pp. 1–45, 2024, doi: 10.7554/elife.92606.3.
M. Parida, S. Sannarangaiah, P. K. Dash, P. V. L. Rao, and K. Morita, “Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases.,” Rev. Med. Virol., vol. 18, no. 6, pp. 407–421, 2008, doi: 10.1002/rmv.593.
N. Tomita, Y. Mori, H. Kanda, and T. Notomi, “Loop-Mediated Isothermal Amplification (LAMP) of Gene Sequences and Simple Visual Detection of Products,” Nat. Protoc., vol. 3, no. 5, pp. 877–882, 2008, doi: 10.1038/nprot.2008.57.
World Health Organization, “Dengue Guidelines for Diagnosis, Treatment, Prevention and Control, Geneva: World Health Organization,” World Heal. Organ., 2009, doi: 10.1590/s0074-02761992000700024.
D. W. Vaughn, S. Green, and S. Kalayanarooj, “Dengue Viremia Titer , Antibody Response Pattern , and Virus Serotype Correlate with Disease Severity,” J. Infect. Dis., vol. 181, no. 1, pp. 2–9, 2000, doi: https://doi.org/10.1086/315215.
A. Srikiatkhachorn and F. K. James, “Endothelial Cells in Dengue Hemorrhagic Fever,” Antivir. Res, vol. 0, no. 0, pp. 160–170, 2014, doi: 10.1016/j.antiviral.2014.07.005.
Downloads
Additional Files
Posted
License
Copyright (c) 2026 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
