Distinction in Differential Leukocyte Count Results in Whole Blood Stored with K3EDTA and Na2EDTA Anticoagulants Using Manual and Automatic Methods
Perbedaan Hasil Differential Leukocyte Count pada Whole Blood yang Disimpan dengan Antikoagulan K3EDTA dan Na2EDTA Menggunakan Metode Manual dan Otomatis
DOI:
https://doi.org/10.21070/ups.11345Keywords:
Differential Leukocyte Count (DLC), Whole Blood, Anticoagulan, Storage Duration, MethodAbstract
Leukocyte Differential Count (DLC) is influenced by pre-analytical factors, including anticoagulant type and sample storage duration. This study aimed to evaluate the effect of K₃EDTA and Na₂EDTA on DLC results using automated and manual methods after storage at 2–8°C. A laboratory experimental study with a repeated-measures design was conducted using whole blood samples from six healthy male subjects aged 17–25 years. Samples were analyzed at 0, 24, and 60 hours using a hematology analyzer and peripheral blood smear microscopy. Statistical analysis showed no significant differences (p > 0.05) in lymphocyte, MID cell, and granulocyte values based on storage duration, anticoagulant type, or analytical method. Although mild leukocyte morphological changes were observed after prolonged storage, these changes did not significantly affect DLC results. Therefore, whole blood samples anticoagulated with K₃EDTA or Na₂EDTA remained stable for Differential Leukocyte Count examination after storage at 2–8°C for up to 60 hours.
Downloads
References
Y. Fuana, U. N. Fidarotul, C. D. Pratiwi, and D. S. Niagrum, “Perbedaan Penggunaan Antikoagulan K3EDTA Konvensional dan Vacutainer Terhadap Kadar Indeks Eritrosit Menggunakan Hematology Analyzer,” Borneo J. Med. Technol., vol. 7, no. 2, pp. 647–652, 2021.
H. Zahraini, Y. N. Indrasari, and H. Kahar, “Comparison of K2 and K3 EDTA Anticoagulant on Complete Blood Count and Erythrocyte Sedimentation Rate,” Indonesian Journal of Clinical Pathology and Medical Laboratory, vol. 28, no. 1. pp. 75–79, 2021. doi: 10.24293/ijcpml.v28i1.1735
U. Sukorini, T. Ratnaningsih, and D. Ikarumi, “The Effect Of Excessive Disodium Ethylene Diamine Tetraacetic Acid (Na2EDTA) Anticoagulant Concentration On Leukocytes Profile In Peripheral Blood Examination,” Med J Indones, vol. 16, no. (3), pp. 168–175. doi.org/10.13181/mji.v16i3.271
L. S. Dewi and D. P. Kusuma, “Perbandingan Hasil Pemeriksaan Hitung Jumlah Eritrosit Menggunakan EDTA Konvensional dan Vacutainer,” Jurnal Surya Medika (JSM), vol. 7, no. 2, pp. 181-184, 2022.
S. T. Wahdaniah, “Perbedaan Penggunaan Antikoagulan K2EDTA dan K3EDTA Terhadap Hasil Pemeriksaan Indeks Eritrosit,” Jurnal Laboratorium Khatulistiwa, vol. 2, no. 2, pp. 21–25, 2018. doi.org/10.30602/jlk.v1i2.147
N. Aunisajidah and M. A. Afriansyah, “Pengaruh Lama Penundaan Spesimen Darah K2EDTA Terhadap Morfologi Leukosit Pada Apusan Darah Tepi,” Prosiding Seminar Nasional UNIMUS, vol. 7, pp. 734–740, 2024.
M. F. Aminuddin, A. Soda, and M. T. Mawardani, “Gambaran Jumlah dan Jenis Leukosit pada pasien Rumah Sakit Umum Daerah Abdul Wahab Sjahranie Samarinda,” Jurnal Teknologi Laboratorium Medik Borneo, vol. 5, no. 1, pp. 47–52, 2025. doi.org/10.35728/jutelmo.v5i1.1866
S. Darmayani, FE Hasan, and D. Ekafitria A, “Perbedaan Hasil Pemeriksaan Jumlah Leukosit Antara Metode Manual Improved Neubauer dengan Metode Automatic Hematology Analyzer,” Jurnal Kesehatan MANARANG, vol. 2, no. 2, pp. 72-75, 2016. doi: 10.33490/jkm.v2i2.18
E. A. Maharani and D. Astuti, “Stability of Routine Hematology Sample Using the Medonic M-Series Analyzer,” Medical Laboratory Technology, vol. 6, no. 2, pp. 108–114, 2020. doi.org/10.31964/mltj.v0i0.305
C. Coutaz, A. Mamin, and K. Mintzas, "Evaluation of the Sysmex XQ-320 Three-Part Differential Haematology Analyser and Its Flagging Capabilities," Journal of Clinical Laboratory Analysis, vol. 38, no. 4, pp. 1–12. https://doi.org/10.1002/jcla.25017
G. Nugraha and N. A. Ningsih, “Penundaan Pemeriksaan Differential Count Terhadap Gambaran Scattergram Hematology Analyzer Cell-Dyn Ruby,” Jurnal Media Analis Kesehatan, vol. 12, no. 1, pp. 9–17, 2021. doi.org/10.32382/mak.v12i1.2036
R. R. Azimata, A. T. Ningrum, and W. Rahmatullah, “Perbedaan Jumlah Leukosit Pada Darah EDTA Segar dan Darah EDTA yang Ditunda Selama 2 Jam,” Jurnal Ilmu Keperawatan, vol. 13, no. 1, pp. 120–130, 2024. doi.org 10.35328/keperawatan.v13i1.2619
A. Irding, F. Landgren, and E. Aardal, “Time-Dependent EDTA Effect on Leukocyte Differential Count and Morphology Compared to Blood Smear Made by Direct Finger Prick,” International Journal of Laboratory Hematology, vol. 47, no. 5, pp. 826–831, 2025, doi: 10.1111/ijlh.14498
N. Ersanto, A. D. Infantria, T. Sundari, A. A. Mandasari, S. Nur, and H. Yusmiati, “Evaluasi Hapusan Darah Tepi Darah K3EDTA Segera Di Periksa dan Ditunda 24 Jam Dalam Suhu 2-8oC,” Jurnal SainHealth, vol. 7, no. 2, pp. 79–84, 2023. doi.org/10.51804/jsh.v7i2.14357.79-84
G. Nugraha, N. A. Ningsih, T. Sulifah, and S. Fitria, “Stabilitas Pemeriksaan Hematologi Rutin Pada Sampel Darah yang Didiamkan Pada Suhu Ruang Menggunakan Cell-Dyn Ruby,” The Journal of Muhamadiyah Medical Laboratory Technologist., vol. 1, no. 4, pp. 21-29, 2021. doi.org/10.30651/jmlt.v4i1.8255
S. U. Ozmen and Y. Ozarda, “Stability Of Hematological Analytes During 48 Hours Stoarge at Three Temperatures Using Cell-Dyn Hematology Analyzer,” J Med Biochem, vol. 40, no. 3, pp. 252–260, 2021, doi: 10.5937/jomb0-27945
E. Kurniasih and T. D. Astuti, "Perbandingan Hasil Hitung Jumlah Sel Darah Spesimen Darah Vena EDTA Menggunakan Metode Manual dan Otomatis (Comparison of Result Counting Blood Cell Number of EDTA Vena Blood Specimen Using Manual and Automatic Methods)," Borneo Journal of Medical Laboratory Technology, vol. 6, no. 2, pp. 495-501, May 2024, doi: 10.33084/bjmlt.v6i2.5876
I. El Savitra and A. Aliviameita, “The Effect of Wholeb lood Storage with K₃EDTA and Na₂EDTA
Anticoagulants on Leukocyte Count, Neutrophil Count, and Leukocyte Morphology,” pp. 1–9. 2025. doi.org/10.21070/ups.8668
V. Roccaforte et al., “Effects of Different Blood Storage Conditions on Leukocytes Cell Population Data Using The Sysmex XN Hematological Analyzer,” Biochemica Medica, vol. 36, no. 2, pp. 1–13, 2026. doi.org 10.11613/BM.2026.020704
Puspitasari, A. Aliviameita, S. D. Y. Wahyudhi, dan F. P. Purwanti, "Stabilitas Sampel Darah Terhadap Profil Hematologi Dengan Metode Otomatis," The Journal Of Muhammadiyah Medical Laboratory Technologist, vol. 5, no. 1, pp. 1–7, 2022, doi: 10.30651/jmlt.v5i1.12667
A. Astrand, C. Wingren, C. Walton, J. Mattsson, K. Agrawal, M. Lindqvist, L. Odqvist, B. Burmeister, S. Eck, G. Hughes, G. Luporini Saraiva, A. Schantz, I. Psallidas, and C. McCrae, “ A Comparative Study of Blood Cell Count in Four Automated Hematology Analyzers : An Evaluation of The Impact of Preanalytical Factors,” PloS ONE, vol. 19, no. 5, pp. 1–19, 2024. doi.org 10.1371/journal.pone.0301845
I. Jamal, “Cytoplasmic vacuolations in peripheral blood smear: A significant finding that is often ignored: An overview,” Indian Journal of Pathology and Oncology, vol. 9, no. 2, pp. 201-203, 2022, doi: 10.18231/j.ijpo.2022.048
A. Aliviameita, P. Puspitasari, W. I. Amilya, and I. El Savitra, “Optimization of Whole Blood Specimen Handling for Complete Blood Count (CBC) Results and Blood Cell Morphology,” Jurnal Kesehatan, vol. 16, no. 2, pp. 285–296, 2025. doi.org/10.26630/jk.v16i2.5126
R. Febriyani and B. Santosa, "Kualitas Makroskopis dan Mikroskopis Morfologi Lekosit pada SADT Berdasarkan Variasi Suhu Pengeringan," Medika Respati: Jurnal Ilmiah Kesehatan, vol. 15, no. 4, pp. 245–252, Nov. 2020.
Downloads
Additional Files
Posted
Categories
License
Copyright (c) 2026 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
