The Effect of Moringa oleifera L. Leaf Extract on MCV, MCH, and MCHC in Male White Rats (Rattus norvegicus) with Hyperlipidemia
Pengaruh Ekstrak Daun Kelor (Moringa oleifera L.) Terhadap Nilai MCV, MCH, dan MCHC pada Tikus Putih Jantan (Rattus norvegicus) dengan Kondisi Hiperlipidemia
DOI:
https://doi.org/10.21070/ups.11342Keywords:
Moringa oleifera, Hyperlipidemia, MCV, MCH, MCHCAbstract
Hyperlipidemia is a metabolic disorder characterized by elevated blood lipid levels that increase oxidative stress and may damage erythrocyte membranes, affecting erythrocyte indices including Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), and Mean Corpuscular Hemoglobin Concentration (MCHC). Moringa leaves (Moringa oleifera L.) from Sidokepung, Sidoarjo, contain bioactive compounds with potential antihyperlipidemic and antioxidant properties. This study evaluated the effect of Moringa leaf extract on MCV, MCH, and MCHC in hyperlipidemic male white rats (Rattus norvegicus). Thirty rats were divided into six groups: negative control, two positive controls, and three treatment groups receiving extract doses of 500, 750, and 1000 mg/kg body weight. Data were analyzed using Two-Way ANOVA. MCV and MCH showed no significant differences between treatment groups (p>0.05). MCHC significantly changed before and after treatment (p<0.05), although no significant differences were observed between groups. Moringa leaf extract significantly affected MCHC but not MCV or MCH.
Downloads
References
H. Z. Himi, M. M. Rahman, S. A. Hasan, L. R. M. D. Cruze, T. S. Zaman, and M. M. Chowdhury, “An Evaluation of Anti-hyperlipidemic Activity of Ethanolic Extract of Moringa oleifera on High Fat Induced Hyperlipidemic Rat Model,” Int. J. Biochem. Res. Rev., vol. 33, no. 4, pp. 33–39, 2024, doi: 10.9734/ijbcrr/2024/v33i4867.
H. H. Stibis, L. Kadir, Z. F. Ahmad, D. Epidemiologi, F. Olahraga, and U. N. Gorontalo, “Analisis faktor resiko terhadap kejadian Hiperlipidemia Pra Lansia,” Jambura J. Epidemiol., vol. 4, no. 2, pp. 12–21, 2025, doi: 10.56796/jje.v4i2.26311.
A. Pareek et al., “Moringa oleifera: An Updated Comprehensive Review of Its Pharmacological Activities, Ethnomedicinal, Phytopharmaceutical Formulation, Clinical, Phytochemical, and Toxicological Aspects,” Int. J. Mol. Sci., vol. 24, no. 3, 2023, doi: 10.3390/ijms24032098.
T. Nurhayati, M. I. Fathoni, S. N. Fatimah, V. M. Tarawan, H. Goenawan, and R. G. Dwiwina, “Effect of Moringa oleifera Leaf Powder on Hematological Profile of Male Wistar Rats,” J. Blood Med., vol. 14, pp. 477–485, 2023, doi: 10.2147/JBM.S407884.
A. M. Galadima, G. E. O., I. Abubakar, S. S. Said, and S. F. Umar, “Effect of Moringa oleifera leaves extract on biochemical, hematological and histopathological parameters in Trypanosoma brucei infected rats,” Biol. Biomed. J., vol. 3, no. 2, pp. 161–171, 2025, doi: 10.21608/bbj.2025.350281.1072.
S. Ghiasi Hafezi et al., “Association between serum hypertriglyceridemia and hematological indices: data mining approaches,” BMC Med. Inform. Decis. Mak., vol. 24, no. 1, p. 410, Dec. 2024, doi: 10.1186/s12911-024-02835-2.
A. D. Ariestiningsih et al., “Moringa Leaf Powder Improves Lipid Profiles and Aortic Thickness in Wistar Rats Model of Prediabetes Mellitus,” Amerta Nutr., vol. 8, no. 2, pp. 278–289, 2024, doi: 10.20473/amnt.v8i2.2024.278-289.
I. Rosidah, S. Ningsih, T. N. Renggani, J. Efendi, and K. Agustini, “Profil Hematologi Tikus (Rattus norvegicus) GalurSprague-Dawley Jantan Umur 7 dan 10 Mnggu,” J. Bioteknol. Biosains Indones., vol. 7, no. 1, 2020, doi: 10.29122/jbbi.v7i1.3568.
W. D. Putri et al., “Aktivitas Daya Hambat dan Elusidasi Golongan Senyawa Ekstrak Daun kelor (Moringa oleifera L.) terhadap Bakteri Staphylococcus aureus ATCC,” SIMBIOSIS XII 138-151 Progr. Stud. Biol. FMIPA UNUD, vol. 50, no. September, pp. 138–151, 2024.
A. Chintya, G. Sijabat, S. Isdadiyanto, and A. J. Sitasiwi, “Histopatologi Hepar Tikus Dengan Induksi Pakan Tinggi Lemak Setelah Pemberian Biji Mahoni ( Rat Liver Histopathology by Inducing High-Fat Diet After Giving Mahogany Seeds ),” J. Ilmu Pertan. Indones., vol. 29, no. 3, pp. 482–490, 2024, doi: 10.18343/jipi.29.3.482.
T. siska W. Achmad Fauzi, Anita dwi Septiarini, “Uji Aktivitas Antiinflamasi Esktrak Sirih Cina ( Peperomia Pellusida ) Pada Tikus Jantan Galur Wistar Yang Diinduksi Karagenan,” J. Inov. Ris. Ilmu Kesehat., vol. 1, no. 3, pp. 282–297, 2023, doi: 10.55606/detector.v1i3.2428.
F. Agustin, A. D. Arinda, Z. S. A. Febrianti, I. K. Rizky, and M. Andriani, “Aktivitas Tepung Tempe Terhadap Kadar Kolesterol Total Tikus Model Hiperkolesterolemia,” Arter. J. Ilmu Kesehat., vol. 6, no. 1, pp. 41–46, 2024, doi: 10.37148/arteri.v6i1.574.
R. Joneri, J. L. Manalu, R. Dewi, and Z. Arieselia, “Penurunan Kadar Malondialdehid (Mda) Pada Tikus Putih Jantan Hiperlipidemia Oleh Pemberian Ekstrak Etanol Bajakah Tampala Reduction Of Malondialdehyde (Mda) Levels In Hyperlipidemic Male White Rats By Administration Of Ethanol Extract Of Bajakah Tampala,” Damianus J. Med., vol. 23, no. 1, pp. 35–41, 2024.
S. I. M. R. Puspitasari, Andika Aliviameita, Anggun Puspita, “Herbal combination therapy of coriander and red ginger extracts to improve hematological profiles in hyperlipidemia : in vivo study,” MEDISAINS J. Ilm. Ilmu-Ilmu Kesehat., vol. 23, no. 3, pp. 138–143, 2025, doi: 10.30595/medisains.v23i3.26399.
Y. Dini Prastyo Wati, Syafrudin Ilyas, “Prinsip Dasar Tikus sebagai Model Penelitian,” USUPress, p. iii+91, 2024.
H. U. Swandono, A. Prodyanatasari, N. Hidayah, A. Laksono, C. Fatia Ulfa, and K. Nisa, “Studi Fitokimia Kualitatif Daun Kelor (Moringa oleifera) Berbasis Reaksi Kimia-Fisika,” J. Sint. Penelit. Sains, Terap. dan Anal., vol. 6, no. 1, pp. 157–168, 2025, doi: 10.56399/jst.v6i1.269.
M. A. Fatimah Siti, S. Rifani Hutami, and C. A. Ike, “Skrining Fitokimia dan Uji Kalitas Aktivitas Antioksidan Ekstrak Etanol Daun Kelor (Moringa oleifera Lam) dengan Metode Kromatografi Lapis Tipis,” J. Farm. dan Sains, vol. 9, no. 1, pp. 114–124, 2025, doi: 10.51997/r7x6dp75.
P. Puspitasari. Andika Aliviameita, “Modul Hematologi patologis,” Umsida Press, 2019.
M. F. D. Arwan Bin Laeto, Rara Inggarsih, Septi Purnamasari and F. I. Taharu, “Analisis Profil Eritrosit Tikus Putih (Rattus norvegicus) Pasca Diet Vegetarian,” J. Ilm. Univ. Muhammadiyah But., vol. Volume 8, no. 2, pp. 312–325, 2022, doi: https://doi.org/10.35326/pencerah.v8i1.1901.
B. Kaptaner, A. Doğan, E. Doğan, and F. Dönmez, “Bisphenol F induces oxidative stress and hematotoxicity in male rats,” J. Basic Appl. Zool., vol. 1, no. 1, pp. 1–10, 2026, doi: 10.1186/s41936-025-00539-1.
A. Bettiol et al., “Erythrocyte oxidative stress and thrombosis,” Expert Rev. Mol. Med., vol. 24, no. e31, pp. 1–26, 2022, doi: 10.1017/ERM.2022.25.
S. Isdadiyanto, A. J. Sitasiwi, and S. M. Mardiati, “Profil Lipid Tikus ( Rattus norvegicus) Hiperlipidemia zsetelah Terpapar Ekstrak Etanol Biji Mahoni ( Swietenia mahagoni ) Lipid Profile of Rat ( Rattus norvegicus L .) Hyperlipidemic After,” Bul. Anat. dan Fisiol., vol. 9, no. 1, pp. 85–92, 2024, doi: 10.14710/baf.9.1.2024.85-92.
N. R. Naufal, D. R. Budiani, N. A. Setyawan, P. S. Kedokteran, F. Kedokteran, and U. S. Maret, “Pengaruh Ekstrak Etanolik Daun Kelor ( Moringa oleifera , Lam . ) Terhadap Ekspresi NF- κβ Pankreas Tikus Wistar ( Rattus norvegicus ) Model Sindroma Metabolik Terinduksi,” Plex. Med. J., vol. 2, no. 5, pp. 188–196, 2023, doi: 10.20961/plexus.v2i5.629.
M. D. Satrianugraha, Y. Naldi, and I. Paridah, “Perbandingan Efektivitas Ekstrak Angkak ( Monascus purpureus ) dan Simvastatin Terhadap Penurunan Kolesterol total , LDL dan HDL pada Tikus Putih Jantan ( Rattus novergicus ) galur sprague dawley dengan hiperlipidemia,” J. Kedokt. dan Kesehat., vol. 4, no. 2, pp. 78–84, 2018, doi: 10.33603/tumed.v4i2.1711.
A. J. Barinda et al., “Statin Mencegah Penurunan Kadar Hemoglobin dan Parameter Hematologi Lainnya pada Model Tikus Gagal Ginjal Eksperimental Statin Prevents the Declining of Hemoglobin Level and Other Hematologic Parameters on the Renal Failure Rat Model,” J. Med. Heal., vol. 3, no. 2, pp. 157–163, 2021, doi: https://doi.org/10.28932/jmh.v3i2.3332.
E. S. Rumaseuw, Y. Iskandar, and E. Halimah, “Hematological Parameters in Subchronic Toxicity Test of Black Garlic Ethanol Extract in Rats,” J. Fundam. Appl. Pharm. Sci., vol. 2, no. 2, pp. 76–85, 2022, doi: 10.18196/jfaps.v2i2.13755.
D. Juliana, K. Simanjuntak, T. Susantiningsih, and D. Purwaningsih, “Literatur Review : Tumbuhan Kelor ( Moringa oleifera ) sebagai Salah Satu Tumbuhan Obat dengan Aktivitas,” J. Med. Lab., vol. 4, no. 2, pp. 79–93, 2025, doi: 10.57213/medlab.v4i2.297.
A. Nining Wahyuni. Andika, “Comparison of Erythrocyte Index Values of Venous and Capillary Blood,” Medicra (Journal Med. Lab. Sci., vol. 4, no. 1, pp. 13–16, 2021, doi: 10.21070/medicra.v4i1.895.
V. Tutino et al., “Peningkatan Signifikan pada Indeks Stres Oksidatif pada Membran Eritrosit Pasien Obesitas dengan Penyakit Hati Berlemak yang Terkait Metabolik,” J. Pers. Med., vol. 14, no. 3, pp. 1–12, 2024, doi: 10.3390/jpm14030315.
C. Indriputri and R. Maulana, “Pengaruh Pemberian Diet Tinggi Lemak Terhadap Profil Lipid dan Gula Darah Puasa Serum Tikus Putih ( Rattus Norvegicus ) Galur Wistar,” Hig. J. Kesehat. Lingkung., vol. 8, no. 3, pp. 144–148, 2019, doi: 10.24252/higiene.v8i3.35819.
I. Karam et al., “Regulation effect of Aspirin Eugenol Ester on blood lipids in Wistar rats with hyperlipidemia,” J. BMC Vet. Res., vol. 11, no. 1, pp. 1–7, 2015, doi: 10.1186/s12917-015-0523-5.
D. I. Nurdin, W. Bodhi, and J. S. Lebang, “Uji efektivitas antihiperkolesterolemia ekstrak etanol Daun kelor (Moringa oleifera Lam) pada tikus putih jantan (Rattus norvegicus),” J. Pharmacon, vol. 10, no. November, pp. 5–10, 2021, doi: 10.35799/pha.10.2021.37417.
Downloads
Additional Files
Posted
Categories
License
Copyright (c) 2026 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
