Antibacterial Activity Assay of Crude Extracts from the Roots and Leaves of Kipait (Tithonia diversifolia (Hemsl.) Gray) Against Staphylococcus aureus and Escherichia coli
Uji Aktivitas Antibakteri Ekstrak Kasar Akar dan Daun Kipait (Tithonia diversifolia (Hemsl.) Gray) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli
DOI:
https://doi.org/10.21070/ups.11336Keywords:
Kipait, crude extract, antibacterial activity, Staphylococcus aureus, Escherichia coliAbstract
The increasing resistance of bacteria to antibiotics has spurred the search for natural antibacterial sources, one of which is Kipait (Tithonia diversifolia (Hemsl.) Gray). This study aims to determine the antibacterial activity of Kipait root and leaf extracts against Staphylococcus aureus and Escherichia coli, as well as to compare their effectiveness. This laboratory experimental study used Kipait roots and leaves macerated in 70% ethanol (1:4 w/v) for 3×24 hours. Secondary metabolites were identified through phytochemical testing, while antibacterial activity was assessed using the Kirby–Bauer disk diffusion method at concentrations of 25%, 50%, 75%, and 100%, with 250 mg of chloramphenicol as the positive control and sterile distilled water as the negative control. The results showed that all extracts were able to inhibit the growth of S. aureus and E. coli. The conclusion of this study is that Kipait leaf extract has potential as a source of natural antibacterial agents.
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