Combination Test of Turi Leaf Extract (Sesbania grandiflora (L.) Pers.) with Vitamin C on SGOT and SGPT as Indicators of Hepatotoxicity in Male Wistar Rats (Rattus norvegicus) Induced by Paracetamol
Uji Kombinasi Ekstrak Daun Turi Putih (Sesbania grandiflora (L.) Pers.) dengan Vitamin C terhadap SGOT dan SGPT sebagai Indikator Hepatotoksisitas pada Tikus Putih Jantan Galur Wistar (Rattus norvegicus) yang di Induksi Parasetamol
DOI:
https://doi.org/10.21070/ups.11269Keywords:
White Turi Leaves, Vitamin C, Paracetamol, SGOT(Serum Glutamic Oxaloacetic Transaminase), SGPT (Serum Glutamic Pyruvic Transaminase)Abstract
Sesbania grandiflora (L.) Pers. leaves contain secondary metabolites, including alkaloids, saponins, steroids, triterpenoids, phenolics, and tannins, with antioxidant potential. Toxic doses of paracetamol generate N-acetyl-p-benzoquinone imine (NAPQI), causing oxidative stress, hepatocyte damage, and increased SGOT, SGPT, and MDA levels. This study evaluated the hepatoprotective effect of a combination of S. grandiflora leaf extract and vitamin C in male Wistar rats induced with toxic-dose paracetamol. A pre-post test control group design was conducted using 40 rats divided into eight groups. SGOT and SGPT were measured enzymatically, while MDA was analyzed using the Wills method. Paracetamol induction significantly increased SGOT, SGPT, and MDA levels, whereas treatment with the extract–vitamin C combination reduced all three parameters. Statistical analysis using the Kruskal–Wallis test for SGOT and MDA and Welch ANOVA for SGPT showed significant differences among groups (p<0.05). These findings suggest that the combination has promising hepatoprotective activity against paracetamol-induced liver injury.
Downloads
References
Q. Chen et al., "Molecular mechanisms in liver repair and regeneration: From Physiology to Therapeutics," Signal Transduction and Targeted Therapy, vol. 12, no. 1, pp. 124, 2025. DOI: 10.1038/s41392-024-02104-8.
A. Hionides-Gutierrez, B. L. Woolbright, and H. Jaeschke, "Novel Emerging Mechanisms in Acetaminophen (APAP) Hepatotoxicity," Liver International, vol. 45, no. 2, pp. 456-468, 2025. DOI: 10.1111/liv.16167.
H. Jaeschke, A. Ramachandran, M. L. Chao, and B. L. Woolbright, "Acetaminophen Hepatotoxicity: Paradigm for Understanding Mechanisms of Drug-Induced Liver Injury," Annual Review of Pathology: Mechanisms of Disease, vol. 19, pp. 405-428, 2024. DOI : 10.1146/annurev-pathmechdis-051122-094016.
Y. Wang et al., "Acetaminophen-induced liver injury: Molecular mechanism and treatments from natural products," Frontiers in Pharmacology, vol. 14, pp. 1122632, 2023. DOI: https://doi.org/10.3389/fphar.2023.1122632.
A. G. Panggayo and J. Rohmah, “Antioxidant Activity of White Turi (Sesbania grandiflora (L.) Pers.) Leaf Extract on the Kidney Organ of White Rats, BUN and Creatinine Parameters Induced by Toxic Doses of Paracetamol,” Universitas Muhammadiyah Sidoarjo, pp. 1–16, 2024. DOI : https://doi.org/10.21070/ups.6781.
E. W. Kusuma and Y. A. M. Agami, “Uji aktivitas hepatoprotektor kombinasi ekstrak etanol daun pandan wangi (Pandanus amaryllifolius Roxb.) dan kayu manis (Cinnamomum burmanii) terhadap kadar MDA yang diinduksi parasetamol,” Jurnal Kesehatan Kusuma Husada, pp. 68–73, Jan. 2020. DOI: https://doi.org/10.34035/jk.v12i1.580.
K. Du et al., "Oxidative stress during acetaminophen hepatotoxicity: Sources, Pathophysiological Role and Therapeutic Potential," Redox Biology, vol. 41, pp. 101899, 2016. DOI: https://doi.org/10.1016/j.redox.2016.10.001.
P. R. Pratiwi, W. Herdwiani, and T. Wijayanti, “Uji aktivitas hepatoprotektor ekstrak etanol dan fraksi n-heksana, etil asetat, dan air umbi bawang dayak (Eleutherine palmifolia (L.) Merr.) pada Tikus Jantan Galur Wistar,” Jurnal Penelitian Kesehatan Suara Forikes, vol.14, no.1, pp. 77–82, Jan. 2023, DOI: https://forikes-ejournal.com/index.php/SF/article/view/sf14116.
Fitria et al., “Ekstrak etanol batang ashitaba (Angelica keiskei Koidz.) sebagai hepatoprotektor tikus putih galur Wistar yang diinduksi parasetamol,” Prosiding Seminar Nasional Diseminasi Penelitian, vol. 3, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia, pp. 97–104, Sept. 2023. DOI: https://ejurnal.universitas-bth.ac.id/index.php/PSNDPV3/article/view/1137.
F. A. Mokhtar, M. Ahmed, A. S. Al Dhanhani, S. E. I. Elbehairi, M. Y. Alfaifi, A. A. Shati, and A. M. Fakhry, "Distribution, Phytochemical Insights, and Cytotoxic Potential of the Sesbania Genus: A Comprehensive Review of Sesbania grandiflora, Sesbania sesban, and Sesbania cannabina," Pharmaceuticals, vol. 18, no. 1, pp. 64, 2025. DOI: https://doi.org/10.3390/ph18010064.
F. A. Ivandah and J. Rohmah, "Antioxidant Activity of White Turi (Sesbania Grandiflora (L.) Pers.) Leaf Extract on the Liver Organ of White Rats, SGOT and SGPT Parameters Induced by Toxic Doses of Paracetamol: Aktivitas Antioksidan Ekstrak Daun Turi Putih (Sesbania grandiflora (L.) Pers.) Terhadap Organ Hati Tikus Putih Jantan (Rattus novergicus) Parameter SGOT dan SGPT yang Diinduksi Paracetamol Dosis Toksik," 2024. DOI: https://doi.org/10.21070/ups.6498.
V. Oktaruliawan and J. Rohmah, "Differences in ALP and Gamma GT levels of Hepar Male White Rats (Rattus norvegicus) Induced by Toxic Doses of Paracetamol by Administration of Turi Leaf Extract (Sesbania grandiflora (L.) Pers.): Perbedaan kadar ALP dan Gamma GT Hepar Tikus Putih Jantan (Rattus norvegicus) yang di Induksi Parasetamol Dosis Toksik dengan Pemberian Ekstrak Daun Turi (Sesbania grandiflora (L.) Pers.)," 2024. DOI: https://doi.org/10.21070/ups.9300.
N. L. D. Octavrina and J. Rohmah, "Acute Toxicity Test of Ethanol Extract of White Turi Flowers (Sesbania Grandiflora (L.) Pers.) Against Kidney Organs (BUN and Creatinine) In Male White Rats (Rattus Norvegicus) Wistar Strain: Uji Toksisitas Akut Ekstrak Etanol Bunga Turi Putih (Sesbania Grandiflora (L.) Pers.) terhadap Organ Ginjal (Bun Dan Kreatinin) pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar," 2024. DOI : https://doi.org/10.21070/ups.2386.
M. Makrufah and J. Rohmah, "Toxicity of White Turi Leaf Extract on Liver Function in Mice: Toksisitas Ekstrak Daun Turi Putih pada Fungsi Hati Mencit," Indonesian Journal on Health Science and Medicine, vol. 2, no. 2, 2025. DOI: https://doi.org/10.21070/ijhsm.v2i2.246.
M. A. Hidayat and J. Rohmah, "White Turi Flower Acute Toxicity Test (Sesbania grandiflora (L.) Pers.) on Organs Kidney Electrolyte Parameters (Na and K) in Male White Rats (Rattus norvegicus) Wistar Strain: Uji Toksisitas Akut Ekstrak Bunga Turi Putih (Sesbania grandiflora (L.) Pers.) terhadap Organ Ginjal Parameter Elektrolit (Na dan K) pada Tikus Putih Jantan (Rattus norvegicus) Galur Wistar," 2024. DOI: https://doi.org/10.21070/ijhsm.v2i2.246.
E. K. Mas'udah, D. Y. N. Hidayati, S. Santoso, W. W. Astuti, A. Malia, and D. W.K. K. Wardani, "The effect of red Turi leaf extract (Sesbania glandiflora L. Pers) on the reduced levels of TNF-α, IL-1β and the number of bacterial colonies on the puerperium Mus Musculus ovarian inoculated by Staphylococcus aureus," 2022. DOI: 10.30574/gscbps.2022.18.2.0085.
D. Novianti, S. Alam, and P. Wati, "Effect of red turi extract on ulcer length and leukocyte count," Int. J. Herbal Med., vol. 11, no. 2, pp. 78-84, 2023. DOI: https://doi.org/10.26740/lenterabio.v12n1.p70-81.
A. Kuttiappan et al., "Hepatoprotective potential of Sesbania grandiflora leaf extract: An integrated in vivo, in vitro and Molecular Docking Approach," J. Ethnopharmacol., vol. 318, pp. 117045, 2024. DOI : https://doi.org/10.1016/j.jaim.2024.101036.
K. Eawsakul and K. Bunluepuech, “Exploring Synergistic Inhibition of Inflammatory and Antioxidant Potential: Integrated In Silico and In Vitro Analyses of Garcinia mangostana, Curcuma comosa, and Acanthus ebracteatus,” Advances in Pharmacological and Pharmaceutical Sciences, vol. 2024, Article ID 8584015, 27 pages, 2024. DOI: https://doi.org/10.1155/2024%2F8584015.
F. P. A. Nabila and J. Rohmah, “Acute Toxicity Test of White Turi Flower (Sesbania grandiflora (L.) Pers.) Ethanol Extract on SGOT and SGPT Parameters in the Liver of Wistar Strain Male White Rats (Rattus norvegicus),” Journal of Pharmaceutical and Sciences, vol. 6, no. 4, pp. 1578–1589, Oct.–Dec. 2023. DOI: https://doi.org/10.36490/journal-jps.com.v6i4.244.
A.Pratiwi, Yusran, Islawati, and Artati, “Analisis Kadar Antioksidan pada Ekstrak Daun Binahong Hijau Anredera cordifolia (Ten.) Steenis,” Bioma J. Biol. Makassar, vol. 8, no. August 2022, pp. 66–74, 2023, DOI: https://doi.org/10.20956/bioma.v8i2.24968.
R. Putriawaty, Uji Karakteristik Formula Gel Peeling Scrub Daun Turi (Sesbania grandiflora L.). Mataram, Indonesia: Universitas Muhammadiyah Mataram, 2021. DOI: https://repository.ummat.ac.id/4136/.
O. T. Mahardani and L. Yuanita, “Efek Metode Pengolahan Dan Penyimpanan Terhadap Kadar Senyawa Fenolik Dan Aktivitas Antioksidan,” Unesa J. Chem., vol. 10, no. 1, pp. 64–78, 2021, DOI: https://doi.org/10.26740/ujc.v10n1.p64-78.
S. Aisyah, A. S. Gumelar, M. S. Maulana, dan R. A. H. T. Amalia, “Identifikasi Karakteristik Hewan Vertebrata Mamalia Tikus Putih (Rattus norvegicus) Berdasarkan Morfologi dan Anatominya,” dalam Prosiding SEMNAS BIO 2023 UIN Raden Fatah Palembang, Palembang, Indonesia, 2023, pp. 484-493. DOI: https://semnas.biologi.fmipa.unp.ac.id/index.php/prosiding/article/download/602/573.
FK UNUD, “Modul Praktikum Penanganan Hewan Coba,” pp. 1–9, 2019. DOI: https://www.coursehero.com/file/157756545/MODUL-PRAKTIKUM-Penanganan-Hewan-Cobapdf/.
J. A. Obernier and R. L. Baldwin, “Establishing an Appropriate Period of Acclimatization Following Transportation of Laboratory Animals,” ILAR Journal, vol. 47, no. 4, pp. 364–369, 2006, DOI: 10.1093/ilar.47.4.364
S. U. Nipanikar, S. S. Chitlange, and D. Nagore, “Pharmacological Evaluation of Hepatoprotective Activity of AHPL/AYTAB/0613 Tablet in Carbon Tetrachloride-, Ethanol-, and Paracetamol-Induced Hepatotoxicity Models in Wistar Albino Rats,” Pharmacognosy Research, vol. 9, Suppl. 1, pp. S41–S47, 2017, DOI: 10.4103/pr.pr_44_17
A. Febrianti and J. Rohmah, “Antioxidant Activity of Turi Bark Extract (Sesbania grandiflora (L.) Pers.) on Liver Organs SGOT and SGPT Parameters in Rats Induced by Toxic Doses of Paracetamol (Aktivitas Antioksidan Ekstrak Kulit Batang Turi (Sesbania grandiflora (L.) Pers.) terhadap Organ Hati Parameter SGOT dan SGPT pada Tikus yang Diinduksi Paracetamol Dosis Toksik),” Preprint, Program Studi D-IV Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Indonesia, 2025. DOI : https://doi.org/10.21070/ups.7857.
S. Patel, S. Patel, A. Kotadiya, S. Patel, B. Shrimali, N. Joshi, T. Patel, H. Trivedi, J. Patel, A. Joharapurkar, and M. Jain, “Age-related changes in hematological and biochemical profiles of Wistar rats,” Laboratory Animal Research, vol. 40, no. 7, 2024. DOI: 10.1186/s42826-024-00194-7
K. A. D. Mukharomah, M. F. Andriansyah, T. Anggraini, A. Banowati, dan F. D. J. Sayekti, “Uji Aktivitas Hepatoprotektif Kombinasi Madu dan Jintan Hitam terhadap Kadar SGOT-SGPT Tikus Putih yang Diinduksi Minuman Bersoda,” Biogenic: Jurnal Ilmiah Biologi, vol. 1, no. 2, pp. 29–35, 2023. DOI : https://doi.org/10.36841/biogenic.v1i2.3771.
Downloads
Additional Files
Posted
Categories
License
Copyright (c) 2026 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
