The Effectiveness of Gracilaria verrucosa Seaweed Extract on Hemoglobin Levels, Hematocrit, and Erythrocyte Count in White Rats with Hyperlipidemia
Efektivitas Ekstrak Rumput Laut Gracilaria verrucosa Terhadap Kadar Hemoglobin, Hematokrit dan Jumlah Eritrosit pada Tikus Putih dengan Kondisi Hiperlipidemia
DOI:
https://doi.org/10.21070/ups.11267Keywords:
Gracilaria verrucosa, Hyperlipidemia, Hemoglobin, Hematocrit, ErythrocyteAbstract
Hyperlipidemia is a metabolic disorder characterized by elevated blood lipid levels that can trigger oxidative stress and affect hematological parameters, including hemoglobin, hematocrit, and erythrocyte count. Gracilaria verrucosa is a species of red seaweed widely cultivated in Indonesia and contains antioxidant compounds. This study aimed to evaluate the effectiveness of Gracilaria verrucosa extract on hemoglobin levels, hematocrit, and erythrocyte count in hyperlipidemic white rats (Rattus norvegicus). This laboratory experimental study employed a pretest-posttest control group design and was conducted from April to May 2026 using 25 male Wistar rats divided into five groups: a negative control group, a positive control group treated with simvastatin at a dose of 0.18 mg/200 g body weight, and three treatment groups receiving Gracilaria verrucosa extract at doses of 25 , 35 , and 45 mg/200 g body weight. Hyperlipidemia was induced using a high-fat diet and 0.01% propylthiouracil (PTU).
Downloads
References
M. Hasan, C. Weidyaningsih, dan N. M. Yasin, “Analisis Persepsi Masyarakat Indonesia Terhadap Hiperlipidemia ‘Studi pada Sosial Media Twitter,’” J. Surya Med., vol. 10, no. 1, hal. 316–324, 2024, doi: 10.33084/jsm.v10i1.5335.
Indonesian Ministry Of Health Development Policy Board, “Indonesian Health Survey (Survei Kesehatan Indonesia) 2023,” Minist. Heal., hal. 1–68, 2023.
R. Mus et al., “Skrining Status Gizi dan Monitoring Kadar Kolesterol Sebagai Upaya Pencegahan Penyakit Degeneratif,” JMM (Jurnal Masy. Mandiri), vol. 9, no. 1, hal. 88–96, 2025.
R. Kosasih et al., “Kegiatan Pengabdian Masyarakat dalam Rangka Edukasi Masyarakat mengenai Hiperlipidemia serta Deteksi Dini Hiperlipidemia dan Kaitannya dengan Kejadian Obesitas,” J. Pengabdi. Kpd. Masy. Sisthana, vol. 5, no. 2, 2023.
D. H. Anzhari, N. K. Titchie, dan A. M. Lubis, “Donor Darah Dengan Hiperlipidemia Berdampak Terhadap Kualitas Darah yang Disumbangkan : Systematic Review,” Media Penelit. dan Pengemb. Kesehat., vol. 34, no. 4, hal. 1062–1073, 2024.
S. Purwaningsih dan E. Deskawati, “Karakteristik dan Aktivitas Antioksidan Rumput Laut Gracilaria sp. Asal Banten,” J. Pengolah. Has. Perikan. Indones., vol. 23, no. 3, hal. 503–512, 2021, doi: 10.17844/jphpi.v23i3.32808.
Arbi Tarmizi, “Analysis of Location Suitability in Lombok Island Waters for the,” J. Media Kult. Indones., vol. 2, no. 2 desember, hal. 190–205, 2022.
Y. Dwipriyono, P. N. Indah, dan S. Sudiyarto, “Analisis Pendapatan dan Fungsi Produksi Rumput Laut (Gracilaria sp.) di Kecamatan Jabon Kabupaten Sidoarjo,” Berk. Ilm. AGRIDEVINA, vol. 9, no. 2, hal. 122–131, 2020, doi: 10.33005/adv.v9i2.2461.
E. Luringunusa, G. Sanger, D. A. Sumilat, R. I. Montolalu, L. J. Damongilala, dan D. V. Dotulong, “Qualitative Phytochemical Analysis of Gracilaria verrucosa from North Sulawesi Waters (Analisis Fitokimia kuatitatif Gracilaria verrucosa Dari Perairan Sulawesi Utara),” J. Ilm. Platax, vol. 11, no. 2, hal. 2302–3589, 2023, [Daring]. Tersedia pada: http://ejournal.unsrat.ac.id/index.php/platax10.35800/jip.v10i2.48777
I. I. J. Rifka Alkhilyatul Ma’rifat, I Made Suraharta, “Efektivitas Ramuan Ekstrak Rempah Sebagai Imunostimulan Terhadap Tikus Putih Jantan Galur Sprague Dawley,” vol. 2, no. 4, hal. 306–312, 2024.
A. Yuliana, R. Wulandari, dan A. Zahra, “Pemberian Ekstrak Sargassum sp . melalui Pakan Komersil Terhadap Nilai Hematokrit dan Diferensial Leukosit pada Ikan Bawal Bintang Trachinotus blochii Provision of Sargassum sp . Extract Through Commercial Feed Against Hematocrit Value and Leukocyte Diffe,” Intek Akuakultur, vol. 5, hal. 36–49, 2021.
N. laras Agustina, “Potensi Ekstrak Polisakarida Ganggang Merah (Gracilaria verucosa) Kajian In Vivo Pada Mencit Hiperkolesterol,” J. Ilm. Pharm., vol. 6, no. 1, hal. 1–9, 2019.
M. Ghazali et al., “Kandungan Proksimat Dan Profil Metabolit Gracilaria sp Pantai Elak-Elak, Pulau Lombok,” Samota J. Biol. Sci., vol. 4, no. 1, hal. 16–22, 2025, doi: 10.29303/sjbios.v4i1.6842.
S. Isdadiyanto, A. J. Sitasiwi, dan S. M. Mardiati, “Profil Lipid Tikus ( Rattus norvegicus L .) Hiperlipidemia zsetelah Terpapar Ekstrak Etanol Biji Mahoni (Swietenia mahagoni),” Bul. Anat. dan Fisiol., vol. 9, no. 1, hal. 85–92, 2024.
M. D. T. Astra, N. Aini, dan Y. R. Bintari, “Pengaruh Metode Ekstraksi (Maserasi, Digerasi, Sokhletasi) Terhadap Aktivitas Antioksidan Rumput Laut Gracilaria verrucosa,” J. Komunita Kedokt. (Journal Community Med., vol. 10, no. 2, hal. 1–7, 2022.
J. S. Kim, C. Choi, dan H. H. Lee, “Changes in the Biological Activities of Gracilaria verrucosa Extracted Using Different Extraction Solvents,” Appl. Sci., vol. 13, no. 22, 2023, doi: 10.3390/app132212314.
D. P. Wati, S. Ilyas, dan Yurnadi, Prinsip Dasar Tikus sebagai Model Penelitian. Medan: USU Press, 2024.
I. Narayani, “Jumlah eritrosit, hemoglobin dan hematokrit pada tikus (Rattus norvegicus) yang diberi seduhan kopi arabika (Coffea arabica),” J. Biol. Udayana, vol. 27, hal. 204–214, 2023.
P. Puspitasari, A. Aliviameita, A. Puspita, S. Ibnu, dan M. Ramadhan, “Herbal combination therapy of coriander and red ginger extracts to improve hematological profiles in hyperlipidemia : in vivo study,” J. Ilm. Ilmu-ilmu Kesehat., 2025.
W. Jin et al., “Hypolipidemic effect and molecular mechanism of ginsenosides: a review based on oxidative stress,” Front. Pharmacol., vol. 14, no. April, hal. 1–21, 2023, doi: 10.3389/fphar.2023.1166898.
H. J. Cha, “Erythropoiesis: insights from a genomic perspective,” Exp. Mol. Med., vol. 56, no. 10, hal. 2099–2104, 2024, doi: 10.1038/s12276-024-01311-1.
K. Gwozdzinski dan A. Pieniazek, “Red Blood Cells in Normal and Pathological States : Redox Reactions of Hemoglobin,” molecules, hal. 1–19, 2026.
W. Yin, R. I. Alwabli, M. W. Attwa, A. F. M. M. Rahman, dan A. A. Kadi, “Simvastatin: In Vitro Metabolic Profiling of a Potent Competitive HMG-CoA Reductase Inhibitor,” Separations, vol. 9, no. 12, 2022, doi: 10.3390/separations9120400.
E. N. G. da Silva Pereira et al., “Simvastatin Improves Microcirculatory Function in Nonalcoholic Fatty Liver Disease and Downregulates Oxidative and ALE-RAGE Stress,” Nutrients, vol. 14, no. 3, 2022, doi: 10.3390/nu14030716.
D. Tristiyanti et al., “Flavonoid Dari Ekstrak Etanol Daun Tempuh Wiyang (Emilia sonchifolia),” vol. 10, no. 1, hal. 1–10, 2025.
R. Masrikhiyah, “Antioxidant Activity and Total Phenolic of Seaweed Gracilaria sp. from Brebes,” J. Pengolah. Has. Perikan. Indones., vol. 24, no. 2, hal. 236–242, 2021, doi: 10.17844/jphpi.v24i2.34020.
D. A. W. Wibowo, D. S. Damayanti, dan M. Herbani, “Pengaruh Frekuensi Aktivitas Fisik Terhadap Kadar Trigliserida Serum Dan Skor Non Alcoholic Steato Hepatitis (Nash) Tikus Betina …,” … Komunitas (Journal …, 2021, [Daring]. Tersedia pada: https://jim.unisma.ac.id/index.php/jkkfk/article/view/13162%0Ahttps://jim.unisma.ac.id/index.php/jkkfk/article/download/13162/10312
D. Dyah Wulandari, S. Ma’at, L. Veterini, D. F. Sari, dan D. D. Wulansari, “Profil Aktivitas Enzim Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), dan Alanine Transaminase (ALT) Pada Tikus Hiperlipidemia yang Diberi Madu Fermentasi Bawang Tunggal,” J. Sains dan Kesehat., vol. 6, no. 1, hal. 51–58, 2025, doi: 10.30872/jsk.v6i1.636.
T. Maruyama, M. Hieda, S. Mawatari, dan T. Fujino, “Rheological Abnormalities in Human Erythrocytes Subjected to Oxidative Inflammation,” Front. Physiol., vol. 13, no. February, hal. 1–11, 2022, doi: 10.3389/fphys.2022.837926.
G. Wistar Diabetik yang Diinduksi Aloksan, A. Maulidia, S. Wahyu Jatmiko, P. Studi Pendidikan Dokter, F. Kedokteran, dan U. A. Muhammadiyah Surakarta Jl Yani, “Pengaruh Kopi terhadap Parameter Darah pada Tikus Putih,” J. Kedokt. dan Kesehatan, vol. 17, hal. 1–9, 2021, [Daring]. Tersedia pada: https://jurnal.umj.ac.id/index.php/JKK
J. Caesario, S. Adi, dan E. Qurnianingsih, “The Effect of Simvastatin and Virgin Coconut Oil (VCO) Combination Therapy as Triglyceride Plasma Lowering Agent in White Dyslipidemic Male Rat (Rattus norvegicus),” Biomol. Heal. Sci. J., vol. 4, no. 2, hal. 85, 2021, doi: 10.20473/bhsj.v4i2.29762.
K. Verma et al., “Simvastatin ameliorates oxidative stress levels in HepG2 cells and hyperlipidemic rats,” Curr. Res. Pharmacol. Drug Discov., vol. 3, no. January, hal. 100088, 2022, doi: 10.1016/j.crphar.2022.100088.
F. D. Oktavia, “Skrining Fitokimia, Kandungan Flavonoid Total, dan Aktivitas Antioksidan Ekstrak Etanol Tumbuhan Selaginella doederleinii,” J. Kim. Ris., vol. 6, no. 2, hal. 141–153, 2021.
R. D. Ayunda dan S. Malita, “Pemanfaatan Senyawa Flavonoid sebagai Antioksidan pada Penderita Hiperkolesterolemia: Studi Literatur,” Unram Med. J., vol. 13, no. 3, hal. 177–187, 2024, doi: 10.29303/jk.v13i3.5388.
K. M. Clapp, M. L. Ellsworth, R. S. Sprague, dan A. H. Stephenson, “Simvastatin and GGTI-2133, a geranylgeranyl transferase inhibitor, increase erythrocyte deformability but reduce low O2 tension-induced ATP release,” Am. J. Physiol. - Hear. Circ. Physiol., vol. 304, no. 5, hal. 660–666, 2013, doi: 10.1152/ajpheart.00635.2012.
D. M. Tipe, “Jurnal Sains , Teknologi dan Kesehatan Kadar Malondialdehyde ( MDA ) Pada Penderita,” Manggala J., vol. 2, no. 3, hal. 511–524, 2025.
Hasan, F. Esther, dan R. Yunus, “Antioxidant Function in Inhibiting Lipid Peroxidation Resistance in Diabetes Mellitus Patients,” Heal. Inf. J. Penelit., vol. 15, no. 2, hal. 1–11, 2023.
B. D. Lechner et al., “The Effects of Cholesterol Oxidation on Erythrocyte Plasma Membranes: A Monolayer Study,” Membranes (Basel)., vol. 12, no. 9, hal. 1–23, 2022, doi: 10.3390/membranes12090828.
M. A. Siahaan, “Studi Perbandingan Kadar Fosfor Dari Fospolipid Membran Eritrosit Normal Dan Eritrosit Penderita Hipertensi,” Univ. Sari Mutiara Indones., vol. 5, no. 1, hal. 2527–712x, 2020, [Daring]. Tersedia pada: http://e-journal.sari-mutiara.ac.id/index.php/ALM
W. D. Latif, M. Aswad, dan M. A. Bahar, “Perbandingan Efektivitas Klinik Simvastatin dan Atorvastatin Terhadap Profil Lipid Darah: Studi Kasus di Rumah Sakit Universitas Hasanuddin,” J. Sains Farm. Klin., vol. 9, no. 1, hal. 34, 2022, doi: 10.25077/jsfk.9.1.34-41.2022.
Listianingsih, “Pengaruh Penggunaan Simvastatin Terhadap Nilai Glomerular Filtration Rate ( Gfr ) Pasien Hiperlipidemia Di Rumah Sakit Umum Daerah ‘ Z ,’” Soc. Clin. Pharm. Indones. J., vol. 6, no. 2, hal. 11–19, 2021.
A. Setiabudi, D. Pringgenies, dan A. Ridlo, “Aktivitas Penangkapan Radikal Bebas DPPH dan Daya Reduksi Ekstrak Gracilaria verrucosa,” JRST (Jurnal Ris. Sains dan Teknol., vol. 4, no. 2, hal. 47, 2020, doi: 10.30595/jrst.v4i2.5761.
P. Krisna, W. Sujana, A. Vanani, R. Salamah, B. Retnoaji, dan N. Wijayanti, “Evaluasi Kadar Lipid Ikan Zebra ( Danio rerio Hamilton , 1822 ) Model Hiperlipidemia Setelah Perlakuan Ekstrak Akuosa Daun Sirsak ( Annona muricata L .) e-JBST V11 Edisi Januari 2026 e-JBST V11 Edisi Januari 2026,” J. Ilm. BIOSAINTROPIS, vol. 11, no. 2, hal. 104–116, 2026.
Downloads
Additional Files
Posted
Categories
License
Copyright (c) 2026 UMSIDA Preprints Server
This work is licensed under a Creative Commons Attribution 4.0 International License.
